By mixing and taking 50 µL from the first column and working sequentially, 1:2 serial dilutions were made in wells in the columns 2–11. To each well, 150 µL of a cell suspension containing ~104 cells was added with a pipetter with 1-cm3 pipette tips. The final concentration ...
The data are presented as 3–4 independent experiments (i.e., average of 3–4 cell passages, with 4 technical replicates (wells) in each passage). 2.5. Statistical analysis To compare differentiated cells to undifferentiated, or exposed to control cells, analysis of variance was performed by ...
Wells in the library storage plates corresponding to hits (>10 S.D. above mean) were picked and retested in duplicate on 384-well plates to validate α-N-acetyl-galactosaminidase activity. Hit identification, cloning, and gene extension DNA from each hit was isolated (Qiagen DNA Miniprep Kit...
Ida B. Wells-Barnett was an American journalist who led an anti-lynching crusade in the United States in the 1890s. She later was active in promoting justice for African Americans and founded (1910) what was possibly the first Black women’s suffrage gro
In the 1901 article ‘Lynching and the Excuse for It,’ anti-lynching activist Ida B. Wells-Barnett argues that Black Americans were lynched not merely as an extralegal means of securing justice, but primarily due to extreme race prejudice on the part of