Single-cell RNA sequencing and functional analyses identified ICAM1 as a metastasis initiator We previously developed dual-optical lentiviral reporter vectors expressing luciferase 2-eGFP (L2G) or luciferase 2-tdTomato (L2T) to transduce tumor cells in PDXs8,16, which enable the flow analysis, sor...
Stimulation with the combination of PDB plus ionomycin induced LFA-l/ICAM-1-dependent homotypic adhesion of tonsil B cells. Adhesion of tonsil B cells in our system induced tyrosine phosphorylation of Pyk-2. Disruption of homotypic adhesion and concomitant inhibition of induction of protein tyrosine ...
5E and F based on the X-ray crystal structure of NADPH binding domain of gp91(PDB3A1F) and the amino acid sequence of NOX4 (NP_056575.1). The ramachandran plot of NOX4 model suggested the good quality of this model. Then, we docked DA to NOX4. The docking score is −5.77 ...
We then inserted this the PNWFDITEP peptide into a position between residues 158 and 162 of VP2 of HRV-14 (PDB ID 4RHV) via adjusting main-chain conformation of the residues 158 and 162 in a manner that the antibody had no steric conflict with the virus capsid. Thereafter, we performed...
When B-were stimulated with the activator PDB and a ionophore, levels, but not LFA-1 levels, increased. Only if these activators were removed after around 24 hr of activation and the were recultured in fresh medium was there an eightfold induction of LFA-1. Such reculturing in fresh ...
方法:从PDB数据库中获取中间亲和力状态的LFA-1/ICAM-1复合物晶体结构(PDB:1MQ8),适当旋转后溶解于6.5×6.8×8.1 nm~3的水框中,按照生理盐水的浓度0.154mol/L加入钠离子和氯离子,使体系成电中性。用NAMD软件对分子体系进行能量最小化和80ns平衡;将水框加大为7.6×8.2×1.41~3并重新进行能量最小化和三组20...
particular, preferred programs are BLASTN and BLASTP, using the following default parameters: Genetic code=standard; filter=none; strand=both; cutoff=60; expect=10; Matrix=BLOSUM62; Descriptions=50 sequences; sort by=HIGH SCORE; Databases=non-redundant, GenBank+EMBL+DDBJ+PDB+GenBank CDS ...
particular, preferred programs are BLASTN and BLASTP, using the following default parameters: Genetic code=standard; filter=none; strand=both; cutoff=60; expect=10; Matrix=BLOSUM62; Descriptions=50 sequences; sort by=HIGH SCORE; Databases=non-redundant, GenBank+EMBL+DDBJ+PDB+GenBank CDS ...
Stimulation with the combination of PDB plus ionomycin induced LFA-l/ICAM-1-dependent homotypic adhesion of tonsil B cells. Adhesion of tonsil B cells in our system induced tyrosine phosphorylation of Pyk-2. Disruption of homotypic adhesion and concomitant inhibition of induction of protein tyrosine ...
In this assay, the protein kinase C-activating phorbol ester PDB and anti-IgM antibodies, as well as the protein kinase inhibitor, staurosporine, were able to induce LFA-1-dependent binding to ICAM-1. This demonstrates that the high avidity state of LFA-1 can be induced by a protein ...