Sections were randomly selected and incubated overnight at room temperature, with primary antibodies listed in Table 1. Primary antibody solutions were prepared with PBS-Triton (25% v/v). To reveal primary antibodies, after rinsing three times in PBS (1x, pH = 7.4), brain sections were...
A final diagnosis of AD neuropathological change was determined using an “ABC” score derived from three separate 4-point scales. We used the modified Aβ plaque Thal score (A0 = no Aβ or amyloid plaques; A1 = Thal phase 1 or 2; A2 = Thal phase 3; or A3 = T...
Heat mediated (100 °C, 24 min) antibody denaturation was performed using Cell Conditioning 2 (CC2) reagent (Cat# 950–223) prior to each cycle of sequential staining in every panel to denature the primary antibody-HRP complex and prevent subsequent chromophores of binding to any residual ...
Furthermore, T18 is part of a standardized antibody panel designed specifically for tau oligomer preparation from brain lysates, underscoring its specificity and reliability in detecting misfolded tau species [124]. Spin columns for isotype controls were prepared in parallel using rabbit IgG (Millipore...
Nissl substance is aggregated and displaced towards the cell membrane, while phosphorylated (antibody 2F11) and non-phosphorylated neurofilament isoforms (SMI-32) accumulate in the cytoplasm of DNs [7]. In contrast, BCs exhibit an opalescent, glassy, eosinophilic cytoplasm devoid of Nissl substance....
The authors also suggested that, in hippocampal sparing AD, tau pathology may begin in the neocortex, since they found a fourfold increase in the amount of neocortical late-stage tau (antibody Ab39 to a conformational epitope in NFTs detecting late-stage tangles [25]) in hippocampal sparing AD...
In order to calculate the percentage of antibody-positive cells, we counted the total number of cells in the region of interest (ROI) and the number of cells stained positive for the marker. ImageJ was used for quantification. To evaluate relative tumor area of Math1-creERT2::SmoM2Fl/+ ...
d Western blotting for PrP (6D11 antibody) after an ultracentrifugation separation of soluble from insoluble isoforms, relative to the total PrP, in the three limbic regions in samples from uninfected controls (UN) and the two disease time points. The bottom panels are Coomassie stains of total...
a Panel of homeostatic microglia which were detected using a P2RY12 antibody. P2RY12 positive microglia showed a highly ramified morphology in all regions. Quantification of P2RY12 positive cells in hippocampus (b), frontal cortex (c), occipital cortex (d) and substantia nigra (e). HPC data...
Sections were blocked in 10% normal goat serum for 1 h at room temperature and were incubated with primary antibody combinations (FN1-CD31, COL6A2-COL4 or FN1-GFAP) overnight at 4 °C in a humidified chamber. Each secondary antibody to the respective primaries was applied for 2 h at ...