Determination-of-IC50 DescriptionIn vitro whole cell assay by [H3] Hypoxanthine uptake assay Procedure[H3] Hypoxanthine uptake assay (with respect to red blood cell culture2019-04-27 16:06 News WIKI 相关搜索 影响IC50的因素 主要是抗体。一般IC50的数值越小,说明你的抗体的特异性能越强。半抑制浓度...
测定药物对生物活性的影响。比如:对于酶抑制实验,可以通过测定底物的消耗或产物的生成来评估酶的活性;对于细胞增殖实验,可以通过细胞存活率分析(MTT assay)和5溴-2'-脱氧尿苷(Brdu)掺入等方法来测定细胞存活或增殖情况。 05 数据处理 将实验结果进行统计分析,绘制...
Inthistypeofassay,asingleconcentrationofradioligand(usuallyanagonist)isusedineveryassaytube.Theligandisusedatalowconcentration,usuallyatorbelowitsKDvalue.Thelevelofspecificbindingoftheradioligandisthendeterminedinthepresenceofarangeofconcentrationsofothercompetingnon-radioactivecompounds(usuallyantagonists),inorderto...
Understand the potential drug-drug interaction (DDI) liabilities of your compounds by using Cyprotex's cytochrome P450 (CYP450) reversible inhibition assay for a range of isoforms.
To investigate the influence of APG on cell viability, the 3-(4, 5-dimethylthiszol-2-yl)-2, 5-diphenytetrazolium bromide (MTT) assay was performed first. 实验目的 A549 cells were treated with APG at indicated concentrations (20-60 μmol/l) for indicated times (6, 12, 24, and 48 ...
2.3IC50 The IC50is the concentration of drug required for 50% inhibition. IC50is an operational term dependent on the assay conditions. IC90or IC99is sometimes used when complete inhibition is required. Calculation of the fractional occupancy shows that IC90concentration is approximately 10-fold gre...
Advantageously, with the subject invention, the determination and use of the multi-time point IC50 test provides an indication of the inactivation rate of a test compound and eliminates trial and error tests associated with the selection of appropriate assay conditions for the second assay conducted ...
Cyprotex's Cytochrome P450 Time Dependent Inhibition IC50 Shift assay is able to identify both reversible and time-dependent inhibitors and discriminate between compounds which cause reversible and irreversible inhibition or both.
If you want to know more regarding the CC50/IC50 Assay services or have any other questions related to the research solutions, please visit Creative Diagnostics at https://antiviral.creative-diagnostics.com. About Creative Diagnostics Headquartered in New York, Creative Diagnostics is a consultin...
膜片钳技术被称为研究离子通道的“金标准”,是研究离子通道的最重要的技术。随着研究的不断深入,目前膜片钳技术已从常规膜片钳技术发展到全自动膜片钳技术和FluxORTM Thallium Assay等,用来研究化合物与离子通道作用的作用机制,也用于新药申报过程中候选药物的毒性评价和先导化合物的结构优...