Type 1 diabetes mellitus (T1DM) is an autoimmune disorder accompanied by activation of auto-reactive B cells, autoantibody production, and consequently insulin-producing beta cell destruction. Some evidences support the value of autoantibodies such as anti-islet cell antigen (IA-2) auto-antibody in ...
IA-2/PTPRNwas found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker. Protocols Product Specific Protocols WB protocol for IA-2/PTPRN antibody 66045-1-Ig Download protocol IHC ...
in preclinical type 1 diabetes (T1D), and to assess the utility of epitope and isotype-specific IA-2 antibody responses as surrogate markers for disease development, we analyzed these antibodies in 34 initially non-diabetic siblings of affected children derived from the “Childhood Diabetes in Finl...
Binding to the 76F monoclonal antibody was specifically inhibited by sera with antibodies to the JM2 epitope but not with antibodies to the adjacent JM1 epitope, indicating that unique epitopes can be distinguished by this approach. 76F mAb has the unique property to distinguish between the two ...
%Objective To investigate the clinical significance of the combined detection of anti-glutamic acid decarboxylase antibody (GAD-Ab), anti-protein tyrosine phosphatase antibody (IA2-Ab) and anti-islet cell antibody (ICA) in the diagnosis of diabetes mellitus.Methods A total of 136 patients with ...
insulin secretion and glucose intolerance. The NOD knockout mice have shown us that neither of these proteins is required for the development of diabetes. Still unanswered, however, is what makes these two proteins, and not the myriad of other -cell proteins, autoantigens in type 1 diabetes....
内容提示: ·病例报告·糖尿病新世界 2015 年 10 月糖尿病新世界DIABETES NEW WORLD糖尿病是由多种因素引起的导致胰岛功能减退、胰岛素抵抗等引发的糖、蛋白质、脂肪、水和电解质等一系列代谢紊乱综合征,以高血糖为主要临床特点,后期可引发肾、眼、足等多种并症,且不可治愈[1]。 糖尿病在分型上有 1 型糖尿...
There is therefore a need of practical methods for the large-scale production of autoantigens that would serve in the diagnosys of type 1 diabetes. The present invention fulfills this need by providing an inexpensive method of producing IA-2ic as a recombinant protein in Escherichia coli with ...
d Immunostaining with the IA-2 N-terminal antibody shows that the level of IA-2 in IA-2-specific RNAi treated NCI-H82 cells was significantly lower than the control cells. Data (mean ± SE) were derived from three independent experiments (in triplicate), and normalized to GAPDH mRNA ...
to the Type 1 diabetes autoantigen GAD65, or prebinding of serum antibodies to protein A Sepharose prior to addition of antigen, increases immunoreactivity detected in serum samples consistent with the trapping on the beads of anti-idiotypic antibodies that block antibody binding to the autoantigen...