Four moles of Triiodide Ion [I3-], one mole of Thiosulfate Ion [S2O32-] and five moles of Water [H2O] react to form twelve moles of Iodide Ion [I-], two moles of Sulfate Ion [SO42-] and ten moles of Deuteron [H+] Reactants Triiodide I...
All complexes were prepared as described above and diluted to 0.2 mg/mL in 20 mM Tris−HCl (pH 8.0) and 50 mM NaCl. Thermal denaturation curves were determined by monitoring the CD value at 218 nm using a 1-mm optical path-length cell as the temperature was raised from 20...
RNA polymerase I (Pol I) specifically synthesizes ribosomal RNA. Pol I upregulation is linked to cancer, while mutations in the Pol I machinery lead to developmental disorders. Here we report the cryo-EM structure of elongating human Pol I at 2.7 Å re
Blocking the import of nutrients essential for cancer cell proliferation represents a therapeutic opportunity, but it is unclear which transporters to target. Here we report a CRISPR interference/activation screening platform to systematically interrogate the contribution of nutrient transporters to support ca...
aureofaciens : It is composed of twelve subunits, each of M 55 kDa, and has a native molar mass of 625 kDa and an isoelectric point at pH 4.2. In addition, its activity is regulated by reversible adenylylation. Mg 2+ and NaCl but not Mn 2+ protected the purified enzyme from thermal ...
(B) Percentage of area in the microscopy field occupied by protein condensates as a function of both the chaperone:Cc molar ratio (10 μM SET/TAF-Iβ) and NaCl concentration. WT, K5A/K7A/K8A and K86A/K87A/K88A Cc variants are at the upper, middle, and lower histograms, respectively...
What is the concentration, in equivalents per liter, of a solution made by dissolving 4.2 g of chromium(III) sulfate pentahydrate Cr2(SO4)3·5H2O in sufficient water to give a total volume of 500 mL? (The molar mass of the hydrate is 482 g) ...
The supernatant was applied onto a self-packaged GST-affinity column (2 ml glutathione Sepharose 4B; GE Healthcare), and contaminant proteins were removed with wash buffer (lysis buffer plus 200 mM NaCl). The fusion protein was then digested with PreScission protease at 18 °C for 2 h. The...
The ethylacetate phase was washed with aqueous solutions of NaHCO3 (5%) and citric acid (5%) in 0.1 M of NaCl, dried with MgSO4 and evaporated to dryness to give 1.7 g of the crude product as a colorless oil. A solution of 1.7 g (3.92 mmol) of Boc-Lys(Boc)-N(EtOH)2, 1.64 ...
Compounds of formula (I) are disclosed which are Fab I inhibitors and are useful in the treatment bacterial infections wherein: R<1> is C1-4alkyl; R<2> is C1-4alkyl; R<3> is -C1-4alkyl, -C0-4alkyl-Ar or -C0-4alkyl-Het; R<4> is -C1-4alkyl, -(CH2)1-4OH, -OC1-4alkyl...