配制k2hpo4nah2po4电泳缓冲液时取03mollk2hpo4300ml和07mollnah2po4700ml混合求缓冲液的i结果一 题目 配制K2HPO4 /NaH2PO4电泳缓冲液时,取0.3mol/L K2HPO4 300ml 和0.7mol/L NaH2PO4 700ml混合,求缓冲液的I 答案 求pH?H2PO4- HPO42- + H+Ka2(H3PO4)=10^(-7.21)=c(H+)*c(HPO42-)/c(H2PO4-...
05M NaH2PO4的生理盐水透析来平衡经浓缩的提取物,并且在 5. OX 90. Ocm G-200SEPHADEX 凝胶柱上进行色谱。收集无效峰,用生理盐水通过透析平衡并且冷冻于_20°C直至使用。用免疫MEDICS NP-1/NP-3和NP-1/NP-4酶免疫测定(EIA) (Immunomedics, Inc.,Morris Plains, NJ)测定提取物中 CEA 的浓度。使用以下方案...
The stoichiometric mole ratio of (NH4)2Fe(SO4)2, I2 and H2SO4 for a maximum theoretical yield is 2:1:1, which will yield Fe2(SO4)3, (NH4)2SO4 and HI in a ratio of 2:1:1. If you combine284.047g (or1moles) of(NH4)2Fe(SO4)2(molar...
OH H3PO4 + RO O H O OH NaClO2, NaH2PO4 RO O OH R= O OH b HO O OH OH OH O O OH O O HO O OH O OH OH OH OH 图 3 化合物 b 的合成路线 Fig. 3 Synthetic route of compound b 2.3 化合物 d 的合成 在 250 mL 烧瓶中加入 KOH(9.00 g,0.16 mol), 乙醇(50 mL).冰浴条件...
DNA extraction The frozen mycelia were macerated using a pestle and mortar, then further ground with the aid of an extraction buffer (50 mM of NaH2PO4, 50 mM NaCl, 500 mM Tris-HCl, 5% SDS, pH 8; 600 μL per culture) and glass beads (1 g, equal amounts of 0.5 and 0.1 mm beads...
(f.c. 15 mM); 650μL glucose 45% (f.c. 0.6%); 100μL 0.5 M EDTA pH 8.0 (f.c. 1 mM)], iMed+ [500mL HBSS 1× with phenol red, 7.5mL HEPES 1 M (f.c. 15 mM), 6.5mL glucose 45% (f.c. 0.6%)], Myelin gradient buffer [prepare 1.5 L of NaH2PO4·H2O (0.78 g/L;...
18、 B (8 mol/L 尿素, 0.1 mol/L NaH2PO4, 0.01 mol/L Tris·Cl, pH 8.0), 加入已用Buffer B平衡的Ni-NTA Agarose, 室温搅拌30 min后上柱。用pH梯度洗脱目的蛋白, 收集洗脱液, SDS-PAGE检测后保存含有目的蛋白的洗脱液。在冷库中(4oC), 按1: 30将目的蛋白洗脱液逐滴加入复性液, 同时不断搅拌以...
For this, the cells were collected by centrifugation at 1448g, medium was completely aspirated, and collected cells were washed twice in Hepes/NaCl buffer (21 mM Hepes (pH 7), 137 mM NaCl, 5 mM KCl, 6 mM glucose, 0.7 mM NaH2PO4) before suspending the cells in 1 ml of the same buff...
NaLi2PO4:Tb3+ phosphor material was prepared by solid state diffusion method using NaH2PO4 and LiOH. The CW-OSL sensitivity of the material (particle size 100–200?μm) was optimised by 1.2?mol% Tb3+ doping and annealing and quenching at 800?°C. Correlation between the traps for TL ...
Shoots of 30-d-old seedlings of 'Nesma' wheat were immersed in 0.1 mM solutions of kinetin, abscisic acid, or one of six mineral salts [0.1 mM Ca(NO3)2, NaH2PO4, K2SO4, KH2PO4, KNO3, or NH4H2PO4] and incubated at 20 and 30掳C with continuous irradiance of 150 渭mol m-2 s-...