gene 15..512 /gene="cspA" promoter 15..81 /gene="cspA" misc_feature 82..121 /gene="cspA" /note="synthetic lac operator sequence" 5'UTR 122..255 /gene="cspA" /note="modified cspA 5'UTR" misc_feature 256..270 /gene="cspA" /note="TEE (translation enhancing element)" misc_...
Deletions in the plasmid pMC874 join the promoter of the km r (kanamycin resistance) gene coding for the enzyme aminoglycoside 3′-phosphotransferase to a promoterless lac operon downstream giving a phenotypic change from Lac →Lac +. They differ from most deletions studied in Escherichia coli, ...
(a)蛋白表达质粒pcas-cas5-3的构建,psc101ori/theoriginfrompsc:来自低拷贝(copy)质粒载体psc101的复制起始点,rep101:基于基因repa控制质粒psc101复制拷贝数,arac/l-arabinoseregulatoryprotein:阿拉伯糖调节蛋白,kanr/kanamycinresistance:卡那霉素抗性,abadpromoter/promoterofthel-arabinoseoperonofe.coli:大肠杆菌中受...
Cyanobacteria are model organisms for photosynthesis and are attractive for biotechnology applications. To aid investigation of genotype-phenotype relationships in cyanobacteria, we develop an inducible CRISPRi gene repression library in Synechocystis sp
Genetic elements on the plasmids: Ptac, IPTG-inducible tac promoter; P11F, 11F constitutive promoter32; Pddh*, a variant of the promoter of ddh gene (Supplementary Data 6); LacO, wild-type lac operator; LacO*, a modified lac operator that binds LacI tightly42; RBS1, a strong RBS AAA...
StringTie [44], and then compared to known gene structures to predict novel gene transcripts (designated as “Novel” in Results section) within the intergenic regions of the reference genome. The novel transcripts were aligned to sequences in NCBI nr database using Blastx [45] (cutoff: ...
The relative gene frequency in isogenic Hfr strains of Escherichia coli K12 has been assayed by P1 transduction in order to evaluate the influence of the integrated F factor on the origin and direction of chromosome replication. Three strains with different sites of integration of the F factor wer...
Over those years, bacteria with similar characteristics were isolated from breweries in Sweden, Finland, Germany, Norway, Japan, Spain, Netherlands, France, and Scandinavia. In 1990, an extensive taxonomic study based on a comparison of the 16S rRNA gene sequence analysis of anaerobic strains, ...
label="lac promoter" /note="promoter for the E. coli lac operon" protein_bind 4316..4332 /label="lac operator" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thio...
In addition to different phage sensitivities, lactococci are also characterized by a rich palette of plasmids, which encode most of their adaptations to the dairy environment, such as the enzymes of the lac operon and the cell wall-anchored caseinolytic protease [6,7••]. Lactococcus is ...