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Construction and characterization of SNP linkage map In total, 4,994 physical RAD loci survived testing against 1:1 segregation expectation, grouping and map calculation, which were assigned onto 31 linkage groups finally (Figure 3, Additional files 1 and 2). These physical RAD loci occupied 4,...
Map Construction (defn do-zipmap [ks vs] (apply hash-map (interleave ks vs))) (clojure.test/testing "Problem 61. Write a function which takes a vector of keys and a vector of values and constructs a map from them. Restrictions (please don't use these function(s)): zipmap." (cloju...
For promoter reporter construction, we amplified the targeted regions from human genomic DNA, and insert them into PGL3-basic plasmid. Regular reverse transcription PCR (RT-PCR) and quantitative real-time PCR (qRT-PCR) Total RNA from cells and homogenized tissues were isolated using the RNA simple...
identified mutations at the expected density inTP53(33 mutations in 32 samples, 68.1%)PIK3CA(12 mutations, 25.5%),CDH1(2 mutations, 4.3%),MLL3(2 mutations, 4.3%) andGATA3(one mutation, 2.1%)8(Fig.1b). We did not observe mutations in six genes (CTCF,MAP2K4,PIK3R1,PTEN,RUNX1andTB...
The change of upland to open water could occur in both urban and non-urban regions. In non-urban regions, new water bodies could be formed due to climate variability or the construction of reservoirs [41]. In urban regions, storm water ponds are often created to drain wetlands for developme...
Construction of mutant libraries is important for future studies. Map-based cloning and mutant analysis of the genes underlying PHS resistance will provide new insights in improving PHS resistance of crop species. It is also necessary to use the available crop genome database that will trigger the...
It is reported that the TOD construction of Shanghai North Station has found many well-known Hong Kong developers in Shanghai landmark business to participate in the program discussion, which is expected to accelerate the landing.据悉,上海北站TOD建设找来了在上海地标商业众多的香港知名开发商参与方案...
59. Determination of the building land area: the horizontal projection area of the construction land location and the land enclosed by the boundary. 59、建筑用地面积的介定:建设用地位置和界线所围合的用地之水平投影面积。 60, the building base area refers to the building area of the first floor ...