Discover BioSpherix's advanced biomedical products for optimal cell and tissue culture. Explore our Hypoxia Chambers Cell Culture solutions for better results.
We describe a chamber that can be used to study the effects of hypoxia on cells in culture. The chamber fits inside a standard cell culture incubator. Hypoxic conditions are created and sustained by flushing a hypoxic gas mixture through an impermeable chamber at defined rates. The fractional ...
美国Biospherix低氧箱(hypoxia cell culture chamber) 仪器简介: 控制精度Z高的低氧培养箱,适合干细胞,神经细胞,心肌细胞等低氧条件下的体外无菌培养。 产品特点: (1)可同时控制箱体内的氧气和二氧化碳浓度,符合细胞体外培养要求; (2)无菌滤膜保证无菌的培养环境; ...
Hypoxia Chamber Hypoxia/Hyperoxia Chamber Offered by COY Laboratory Products PreviousNext Description Cells respond to a variety of environmental conditions and having options to apply these varying conditions in vitro is essential. At Applied Biophysics, we encourage different avenues of manipulating the ...
Cell scrapers Cell culture grade PBS 1x Laemmli Sample Buffer Microcentrifuge tubes CoCl2 (necessary for CoCl2 treatment only) Microprobe sonicator Hypoxic Protocol (You will need a tissue culture chamber or hypoxic incubator for this protocol. Standard tissue culture conditions of temperature and humidit...
advances in programmable gas controlled workstations. The ability to replicate accurate and specific gas levels gives scientists the tools to perform research in conditions comparable with those found in vivo. Our hypoxia chamber is the most recent addition to our complete line of atmospheric work...
The cells were cultured in either a normoxic cell incubator (21% O2) or a hypoxia incubator chamber (1% O2) for 24 h. In light-treated groups, cells were exposed to a 660 nm laser (100 mW/cm2; 5 min) and cultured for 12 h. After these treatments, the cell medium was...
The CO2 concentration in each chamber, as well as the temperature and humidity, were monitored continuously. Mice were exposed to gas treatment continuously for 24 hours per day, 7 days a week. The chambers were briefly opened three times per week to monitor health status, provide food and ...
The antibodies used for immunoblotting were an anti-p70 S6 kinase (49D7), phospho-p70 S6 kinase (Thr389) and TSC1 (D43E2) from Cell signaling. An anti-β-actin antibody (sc47778) was purchased from Santa Cruz. Immunofluorescence staining Equal amounts of PASMCs were seeded in chamber ...
For hypoxia treatment, preadipocytes were maintained in a hypoxia chamber (Invivo2 400, Ruskinn Inc.), and the media were replaced every other day inside the chamber. In this study, normoxia was considered as the ambient atmosphere containing 21% O2 and hypoxia, 1% O2. Deferoxamine mesylate ...