The concentration for selection ranges from 100-1000 µg/ml (typically 200 µg/ml) and should be optimized for each cell line. Formula: C20H37N3O13 FW: 527.5 g/mole 货号产品名称规格单价(CNY)数量 10687010 Hygromycin B (50 mg/mL) Each 2,948.00 Table 1 - Selection agents and ...
货号:10687010 规格 描述 C20H37N3o1 式量:527.5 g/mol 浓度:50 mg/mL 潮霉素 B 是一种氨基糖苷类抗生素,可通过中断易位并增加 80S 核糖体上的错误翻译来抑制蛋白质合成。 因为其作用方式与 Geneticin® 或 Zeocin™ 不同,所以可用于双选择实验。 大肠杆菌潮霉素抗性基因(hyg 或 hph)提供对潮霉素 B 的抗性。
On the next day, remove medium and add fresh medium with varying concentrations of Hygromycin B (0, 100, 200, 400, 600, 800μg/mL final concentration). 5. Examine viability every 2days. Culture for 14days. Replace the media containing antibiotics every 3days. ...
Working concentration: 50 - 1,000 µg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 µg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used. ...
Gibco™ Hygromycin B is used as a bacterial selection antibiotic in the concentration range of 200–500 μg/mL. This product is supplied as a 50 mg/mL solution.Resistance to Hygromycin B is conferred by the E. coli hygromycin resistance gene (hyg or hph). Hygromycin B has a different ...
Hygromycin B Gold is normally used at a concentration of 200 µg/ml, a 500‑fold dilution from the stock solution. However, the optimal concentration needs to be determined for your cells. Suggested concentrations of Hygromycin B Gold for selection in some examples of mammalian cells are ...
植物转化 (1,2) 和哺乳动物转染系统 (3-5) 应用参考文献可见于下文。 规格 Validated Application: Eukaryotic Selection⁄Stable Cell Line Generation Agent: Hygromycin Form: Liquid Reagent Type: Antibiotic (Selective) Product Size: 20 mL Concentration: 50 mg⁄mL Shipping Condition: Room Temperature...
Working concentration: 50 - 1,000 µg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 µg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used. Contents Solution, 50 mg...
• Working concentration: 50 - 1,000 μg/ml in cell culture. A commonly used concentration for the selection of mammalian cells is 200 μg/ml. However, the optimal concentration must be experimentally determined, since it may vary depending on the type of cell used. ...
Validated Application: Eukaryotic Selection⁄Stable Cell Line Generation Agent: Hygromycin Form: Liquid Reagent Type: Antibiotic (Selective) Product Size: 20 mL Concentration: 50 mg⁄mL Shipping Condition: Room Temperature目录号:10687010 品牌:ThermoFisher /Invitrogen/ Gibco/ ABI 规格:20 ML # 50540...