M. Hydroxyl radical probe of the calmodulin-melittin complex interface by electrospray ionization mass spectrometry. J. Am. Soc. Mass Spectrom. 16, 225-233 (2005).Wong, J. W.; Maleknia, S. D.; Downard, K. M. Hydroxyl Radical Probe of the Calmodulin-Melittin Complex Interface by E...
Regardless, if the ozone kinetics are known (using the schemes described above), then the hydroxyl radical kinetics can be determined using a competition kinetics approach. The most widespread hydroxyl radical probe compound for ozone-based systems is pCBA. Salient information on pCBA can be found ...
The hydroxyl radical is a useful probe for studying the shape of the surface of a DNA molecule. Using this technique, fine details of DNA structure can potentially be revealed. This unit describes how to use the hydroxyl radical to generate a random cleavage pattern at the surface of the ...
"The Use of para-Chlorobenzoic Acid (pCBA) as an Ozone/Hydroxyl Radical Probe Compound." Ozone: Sci. Eng. 27: 431-436.Y. Z. Pi,J. Schumacher,M. Jekel.The Use of para-Chlorobenzoic Acid (pCBA) as an Ozone/Hydroxyl Radical Probe Compound. Ozone: Science & Engineering . 2005...
Hydroxyl radical (HO•) is a short-lived free radical, and it is the most potent oxidizing transient among the reactive oxygen species. It is an effective, nonselective and strong oxidant that is ubiquitously formed in natural sunlit surface waters (rivers, lakes and seawater and so on), ...
(H2DCFDA, D399). APF and HPF show limited non-selective reactivity and relatively high resistance to light-induced oxidation. Both of these new fluorescein derivatives are nonfluorescent until they react with the hydroxyl radical or peroxynitrite anion; APF will also react with the hypochlorite ...
The hydroxyl radical can be used as a highly sensitive chemical probe to study the structure of DNA (Tullius, 1989) and DNA complexed with proteins (Tullius & Dombroski, 1986; Tullius et al., 1987) or drug molecules (Churchill et al., 1990). The hydroxyl radical cleaves the DNA strand ...
Chemical footprinting of proteins using the hydroxyl radical as a probe is a well characterized method for investigating protein-ligand interfaces. One current method, in which the radicals are formed by millisecond irradiation with synchrotron light, can only be performed at the National Synchrotron Li...
Tumor response to radiotherapy or ferroptosis is closely related to hydroxyl radical (•OH) production. Noninvasive imaging of •OH fluctuation in tumors can allow early monitoring of response to therapy, but is challenging. Here, we report the optim
Hydroxyl radical footprintingFast photochemical oxidation of proteinsProtein structureProtein foldingElectrospray ionisation-mass spectrometryLiquid chromatography–mass spectrometryCharacterisation of the conformational states adopted during protein folding, including globally unfolded/disordered structures and partially ...