This chapter provides an overview on hydroxyl radical footprinting. Hydroxyl radical footprinting of DNA has evolved over the last several years into a facile and powerful technique for studying DNA structure and complexes of DNA with other molecules. The chapter describes the chemistry and history ...
... 结构 Structure 氢氧化放射性足印法 Hydroxyl Radical Footprinting ... www.myoops.org|基于1 个网页 3. 羟自由基足纹法 羟自由基足纹法(Hydroxyl radical footprinting)即是其中应用较广泛的代表 [18] 。 羟游离基足纹法是高分辨率的描绘DNA-蛋白 质…www.docin.com|基于1 个网页 更多释义...
Hydroxyl radical footprinting has been utilized for over 30 years in the study of nucleic acids and their interactions (Burkhoff and Tullius, 1987; Tullius and Dombroski, 1986). Hydroxyl radicals cleave nucleic acids by abstracting a hydrogen atom from the ribose (Hertzberg and Dervan, 1984), ...
time-resolved footprintingRNA footprinting by hydroxyl radical cleavage provides 'snapshots' of RNA tertiary structure or protein interactions that bury the RNA backbone. Generation of hydroxyl radicals with a high-flux synchrotron X-ray beam provides analysis on a short timescale (5-100 msec), ...
II. Development of next-generation hydroxyl radical footprinting. [D] . Xu, Long. 2009 机译:对DNA的氧化损伤:I.分离与识别氧化损伤的DNA有关的蛋白质。二。下一代羟基自由基足迹的发展。 6. Hydroxyl Radical Dosimetry for High Flux Hydroxyl Radical Protein Footprinting Applications Using a Simple ...
We report an integrated workflow that allows mass spectrometry-based high-resolution hydroxyl radical protein footprinting (HR-HRPF) measurements to accurately measure the absolute average solvent accessible surface area () of amino acid side chains. Thi
MonitoringEquilibrium ChangesRNA StructurePeroxidativeOxidativeHydroxyl Radical FootprintingRNA MoleculesGenetic InformationTertiary StructuresBiologic RoleRegulatorBinderCatalystNucleic AcidsPhosphodiester BackboneSingle Nucleotide ResolutionIntermolecular Contact SurfaceEquilibrium TransitionsKinetic TransitionsSolution Var...
The hydroxyl radical can be used as a highly sensitive chemical probe to study the structure of DNA (Tullius, 1989) and DNA complexed with proteins (Tullius & Dombroski, 1986; Tullius et al., 1987) or drug molecules (Churchill et al., 1990). The hydroxyl
5) hydroxyl radical footprinting 羟游离基足纹法 6) free carbon 游离碳 1. Study on removingfree carbonof nanosized WC by heat processing in flowing hydrogen atmosphere; 纳米WC粉氢处理脱除游离碳的研究 2. Determination of silicon carbide andfree carbonin deoxidizers by infrared absorption method wit...
5) hydroxyl radical footprinting 羟游离基足纹法 6) hydroxyl radical 氢氧游离基;氢氧自由基;羟基 HR 补充资料:羟游离基足纹法 分子式: CAS号: 性质:利用OH—来打开DNA主链而获得足纹。用此法发现了用一般足纹法未能查明的接触位点,是利用Fe2+与EDTA的配位化合物促使过氧化氢还原而成的。反应生成的OH—作...