The protocol has been modifiedsuch that it can be applied to either low-buffering or high-buffering capacity samples, both typically encountered during purification protocols using CHT.Esteban FreydellLarry CummingsMark Snyder
EconoFit CHT XT, 40 μm Column, 1 x 1 ml 12009261 Pkg of 1, 1 ml, prepacked mixed mode column, 7 mm x 25 mm, max. pressure 72 psi, for use with NGC Systems or any chromatography system 列表价格: 查询 数量: 添加到报价 添加到热点列表 CHT™ Ceramic Hydroxyapatite XT, ...
column equilibration for 5 min.Elution was performed using a linear gradient of 10±400 or 600 mM for 30 or 50 min while monitoring conductivity.DNA fragments eluted from the column were identi®ed by polyacrylamide gel electrophoresis using Mini-PROTEAN II (Nippon Bio-Rad Labora-tories(Tokyo)...
Absorbances at 490 nm were measured using a microplate reader (Bio-Rad Lab, Hercules, CA, USA). LDH leakage was expressed as the percentage (%) of the total LDH activity, where the total LDH activity of the cells was determined by exposing them to 0.2% (v/v) Triton X-100. ...
DNA was eluted by adding ethanol and centrifuged in a mini spin column and extracted DNA quantified by spectropho- tometer at γ = 260 nm, using the NanoDrop® ND-1000 (Nanodrop Technologies Inc., Wilmington, DE, USA). Histochemical staining was done to evaluate the retention of ...
A 5-ml column of ceramic hydroxyapatite type II (Econo-Pac HPT hydroxyapatite cartridge) with a particle size of 20 μm was obtained from BioRad (Richmond, CA, USA). For immobilized metal-ion affinity chromatography (IMAC), a Fractogel Chelat 650 (s) gel from Merck (Darmstadt, Germany) ...
Tsuru et al., (1988) “A rapid method for the isolation of functional human T lymphocytes using hydroxyapatite column fractionation,” J. Immunological Methods, 106, 169-174. Macro-PrePR Ceramic Hydroxyapatite, BIO-RAD Data Sheet C-100. ...
Briefly, samples were added to 1 mL Wizard DNA Clean-Up Resin and transferred to a syringe barrel with a minicolumn. The bound DNA was pushed through the minicolumn with a syringe plunger. The column was then washed with 2 mL isopro- panol (80%) and transferred to a microcentrifuge ...
Packing and Slurry-in-Place Procedures for CHT™ Ceramic Hydroxyapatite Using a Bio-Rad® InPlace™ Process-Scale Chromatography ColumnSimilarly to the slurry-in-place procedure, unpacking wasperformed by initiating upflow at 100 cm/hr while the toppiston was raised. Once the bed was ...
Fractions 26-34 (green bar in Figure 1) were pooled andloaded onto a Bio-ScaleTM CHT2-I column (catalog #751-0021) previously equilibrated with 5 mM sodium phosphate buffer, pH 6.8. Horse IgGT was eluted with a linear gradient of 0-1 M NaCl, as described in Figure 3.Bio-Rad ...