Journal of Inorganic BiochemistryChaves S, Canario S, Carrasco MP, Mira L, Santos MA. Hydroxy(thio)pyrone and hydroxy(thio)pyridinone iron chelators: physico-chemical properties and anti-oxidant activity. J Inorg Biochem 2012;114:38e46.
The samples were incubated for 15 min and then 75 µL of acetylthiocholine iodide (AChI) solution (16 mM) was added. The reaction was monitored for 5 min at 405 nm. Assays were run with a blank containing all the components except AChE, which was replaced by HEPES buffer. The rates...