Methods: Wash inclusion body effectivity, use Hydrophobic Interaction Chromatography to carry on the purification. 方法:对包涵体进行有效洗涤,采用疏水层析进行纯化。 terms.shengwuquan.com 2. UGRh2GT was purified by ammonium sulfate precipitation, hydrophobic interaction chromatography and ion exchange chromatog...
hydrophobic interaction chromatography 英[ˌhaidrəuˈfəubik ˌɪntərˈækʃən ˌkrəuməˈtɔɡrəfi] 美[ˌhaɪdrəˈfobɪk ˌɪntɚˈækʃən ˌkroməˈtɑɡrəfi] 释义 [医]疏水作用色谱 ...
Hydrophobic interaction chromatography (HIC) is a commonly used technique that exploits these hydrophobic features of proteins as a basis for their separation even in complex biological mixtures (Queiroz et al., J Biotechnol 87:143-159, 2001; Eisenberg and McLachlan, Nature 319:199-203, 1986). ...
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POROS hydrophobic interaction resins Thermo Scientific POROS hydrophobic interaction chromatography (HIC) resins are based on the 50 μm POROS base bead, utilizing a novel coating procedure to enable functionalization with unique hydrophobic ligands. These resins are suitable...
Hydrophobic interaction chromatography (HIC) is a widely used chromatographic technique that finds application at different stages of downstream processing. It was first described by Shepard and Tiselius in 1949 using the term “salting out chromatography.” The name HIC was later coined by Hjerten (...
疏水作用层析(Hydrophobic Interaction Chromatography,HIC)是根据分子表面疏水性差别来分离蛋白质和多肽等生物大分子的一种较为常用的方法。蛋白质和多肽等生物大分子的表面常常暴露着一些疏水性基团,我们把这些疏水性基团称为疏水补丁,疏水补丁可以与疏水性层析介质发生疏水性相互作用而结合。不同的分子由于疏水性不同,它...
Hydrophobic interaction chromatography uses a high salt concentration to induce an interaction between hydrophobic regions of a protein and a weakly hydrophobic column packing. From:Encyclopedia of Separation Science,2000 Also in subject areas: Agricultural and Biological Sciences ...
疏水相互作用层析Hydrophobic Interaction Chromatography 热度: a hydrophobic interaction chromatography strategy for purification of inactivated foot-and-mouth disease virus:一种疏水性灭活口蹄疫病毒相互作用色谱纯化策略 热度: Hydrophobic interaction and reversed phase chromatography ...
Hydrophobic interaction chromatography (HIC) is a versatile method for the purification and separation of biomolecules by using the function of hydrophobicity. The proteins containing both hydrophilic and hydrophobic regions are applied to a HIC column under specified salt buffer conditions, w...