Illustration of the chemical structure of adenosine triphosphate (ATP)4- anion; Increase in the electron-acceptor properties of ATP; Energy diagram of the ATP hydrolysis reaction.doi:10.1007/s10628-005-0022-xK. K. KalninshYu. V. Medvedev...
Conformations of Bcs1L undergoing ATP hydrolysis suggest a concerted translocation mechanism for folded iron-sulfur protein substrate Jingyu Zhan, Allison Zeher, Rick Huang, Wai Kwan Tang, Lisa M. Jenkins & Di Xia Nature Communications volume 15, Article number: 4655 (2024) Cite this article 23...
Three approaches, ATP hydrolysis assays, limited proteolysis protection, and equilibrium dialysis, provide evidence that the amino-terminal domain of Mlh1 binds ATP with >10-fold higher affinity than does the amino-terminal domain of Pms1. This is consistent with a model wherein ATP may first ...
Answer to: Mitochondria are called the 'powerhouse' of the cell because their main function is: a. generating ATP. b. producing lipids. c...
The crystal structure of the MJ0796 ATP-binding cassette, a member of the o228/LolD transporter family, has been determined at 2.7-Å resolution with MgADP bound at its active site. Comparing this structure with that of the ATP-bound form of the HisP ATP-binding cassette (Hung, L. W...
Yongqi Dong1,Yi Zhang1, Han Xu1, Bin Hong1, Yuanjun Yang1, Liangbin Li1, Wenhua Zhang2 & Chen Gao1 Instability of emerging perovskite organometallic halide in humidity environment is the biggest obstacle for its potential applications in solar energy harvest and ...
Schematic diagram of three-filament system with random hydrolysis, where the switching ATP/GTP → ADP/GDP occurs randomly at any ATP/GTP subunit.Dipjyoti DasDibyendu DasRanjith Padinhateeri
Protein at 1 nm to 2 μm was incubated with 4.35 μm to 2 mm ATP in assay buffer supplemented with 5 μm ZnCl2 at 37 °C. Four volumes of Biomol Green reagent were added followed by incubation and absorbance measurement at 620 nm. The difference in absorbance after 15 min of ATP ...
coli phage shock protein PspF and the nitrogen regulation protein NtrC) help transcend the energy barrier at the expense of ATP hydrolysis, leading to full DNA melting in the RPO [34], [35], [36]. These properties mimic some eukaryotic Pol II characteristics, and distinct RPIs of σ54-...
For experiments using nucleotide-free eEF1A, the total ATP concentration was increased to 2 mm to control for possible nonspecific charge effects of nucleotides on the binding of eEF1A to F-actin (36). Actin filaments and bound eEF1A were pelleted by centrifugation at 125,000 × g for ...