Integrating immunome, tissue microarray and human-human hybridoma technology to develop natural human apoptosis-related antibodies免疫技术组织激阵列人-人杂交瘤技术综合天然人凋亡相关抗体单抗制备Despite significant advances in monoclonal antibodies (Mabs) technology over the last two decades, technology to ...
9.3.1Hybrid hybridoma (quadroma technology) First documented work in 1961 about the generation of a hybridized BsAb comprising simultaneously two different antigen-binding domains ofmonoclonal antibodies. Before time, the generation ofbsAbswas performed by quadroma technology (a somatic amalgamation ofhyb...
In this study, we developed novel anti-PSA ... P Nurmikko,V Visnen,T Piironen,... 被引量: 0发表: 2000年 Production, Establishment and Characterisation of Monoclonal Antibody Against Breast Cancer Cell Line (Mcf-7) Ever since hybridoma technology was introduced by Kohlerand Milstein in the...
By this procedure, Milstein and Kohler developed a hybridoma that made a specific antibody . 米尔斯坦和科勒用此方法培养出一个杂种瘤,后来成为一种特效抗体。 www.jukuu.com 3. Hybridoma cells stably secreting antiOVA66 monoclonal antibodies were prepared by hybridoma technology. 采用经典的杂交瘤技术制备...
Hybridoma Technology - A Biotechnology Technique Introduction: Hybridoma technology is used to produce a hybrid cell. These hybrid cells are produced by...
TS Shaughnessy,F Kargi - 《Enzyme & Microbial Technology》 被引量: 21发表: 1990年 Suppressor T-Cell Hybridoma with a Receptor Recognizing KLH-Specific Suppressor Factor Immune responses are known to be regulated by complicated interactions of T-cell subsets that play different functional roles. (...
To introduce megabase-sized segments of DNA into mice, we have developed an alternative strategy utilizing a human chromosome as a vector for transgenesis9. Using this technology, two transmittable human chromosome fragments, one containing the Ig heavy chain locus (IGH, ≈1.5 Mb) and the ot...
Interestingly, the fusion of two immunoglobulin-producing myeloma cells (Cotton and Milstein 1973) was described even before the seminal publication of monoclonal antibody technology (Khler and Milstein 1975). This early experiment showing expression of both parental immunoglobulin genes in the hybrid ...
By contrast, antibodies generated using recombinant expression technology may be manufactured as products of more homogenous monoclonal composition, increasing reproducibility [11]. Whether the DNA sequence encoding the antibodies was recloned from a B cell or hybridoma cell line, or discovered in a ...
was developed and applied to produce human MoAb. In this fermentor, maximum cell density of a heterohybridoma reached 1.2×107cells/ml and MoAb was continuously accumulated at a constant rate for at least 40 days; this led to the production of more than one gram of human MoAb using a ...