The fluorescence emitted by bindinghybridization probescan be measured during either the annealing or the extension phase. The use of these probes allows amplified fragments to be analyzed by performingmeltingcurves, and this is the main advantage overhydrolysisprobes. The amount of fluorescent signal ...
HYBRIDIZATION PROBETomoyasu SUGIYAMAToshio OTAShizuko ISHIIAi WAKAMATSU
必应词典为您提供hybridization-probe的释义,网络释义: 杂交探针;杂合探针;杂交用探针;
Thehybridization probemethod uses the physical property offluorescence resonanceenergy transfer (FRET) and twooligonucleotide probesthat anneal adjacently to the target. Fluorescent dyes are attached to the 3′ end of the upstream probe and the 5′ end of the downstream probe (Figure 4-8A). In ...
Hybridization Probedoi:10.1007/978-1-4020-6754-9_7985A radioactively or by fluorochrome (e.g., biotin) labeled nucleotide sequence that hybridizes with the complementary nucleotide sequences and identifies the homologous tract(s), either on an......
hybridization probe: 来自 EBSCO 喜欢 0 阅读量: 27 作者: RHJ Schlegel 摘要: A definition of the term "hybridization probe," which refers to labeled nucleic acid molecule used to detect complementary DNA sequences after hybridization, is presented. 年份: 2003 ...
What is probe hybridization? Hybridization in Molecular Biology: Hybridization in molecular biology is the process of annealing two separate pieces of DNA or RNA together. This can be used in many types of molecular biology experiments, including those that detect a sequence of interest using probes...
...长度的DNA分开,再经过印迹(blotting)、探针杂合(probe hybridization)等过程,便会呈现厚薄不一的条纹,这些条纹便称为D… mypaper.pchome.com.tw|基于2个网页 3. 探针杂交技术 4.此外,亦可利用聚合酶链反应(PCR)、探针杂交技术(probe hybridization)及病毒之单株抗体来检查是否有病毒之污染。 … ...
Livak, K.J., Flood, S.J.A., Marmaro, J., Giusti, W. and Deetz, K. 1995. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.PCR Methods Applic.4: 357–362. ...
To characterize the phenotypic profile of theSpDgroup relative to other lineages, we measured the growth of 229 strains, including 12SpDstrains, in 24 growth conditions that probe a diversity of potential metabolic performances. These conditions comprise various carbon sources, nitrogen sources, chemical...