Improvedhybridization methods for mRNA detection in tissues have been developed based on the hybridization chain reaction (HCR). We show thatHCR methods can be used for the detection of microRNAs in tissue sections from mouse retinas.HCR can be used for the detection of two microRNAs simultaneously...
hairpin DNA 3 hybridized with hairpin DNA 2 to start the hybridization chain reaction. In this detection strategy, the hybridization chain reaction was triggered by miRNA hybridization and release, which make the target miRNA recycled many times, resulting in the designed biosensors achieving high ...
Hybridization chain reaction (HCR)doi:10.1002/9783527678679.dg05831This article has no abstract.American Cancer SocietyThe Dictionary of Genomics, Transcriptomics and Proteomics
T. Choi研究团队应用杂交链式反应开发出一种多重、等温、杂交连锁反应扩增信号的技术HCR(Hybridization Chain Reaction)(图3)[11][12][13]。借用标准荧光成像设备在整个斑马鱼胚胎中实现了4个目标mRNA的同步原位杂交。RNAscope公司及苏黎世大学的Thomas Stoeger和Lucas Pelkmans团队应用体外生成的分支DNA长链逐级杂交的...
In this study, an electrochemical biosensor was developed based on nanomaterials and the hybridization chain reaction (HCR) for highly sensitive and selective detection of the target miR-378 using K3Fe(CN)6/K4Fe(CN)6 as a redox indicator. MiR-378 is located on human chromosome 5q32 and ...
杂交链反应的意思 杂交
and hybridization chain reaction. Initially, a sandwiched DNA probe is formed between the immobilized capture probe on the sen- sor and the detection probe with the initiator strand. Then, the carried initiator strand triggers a cascade of hybridization events ...
isHCR allows multiplexed, sensitive detection of immunostained proteins in cultured cells, as well as in dense and cleared tissue. Immunosignal hybridization chain reaction (isHCR) combines antibody–antigen interactions with hybridization chain reaction
isHCR allows multiplexed, sensitive detection of immunostained proteins in cultured cells, as well as in dense and cleared tissue. Immunosignal hybridization chain reaction (isHCR) combines antibody–antigen interactions with hybridization chain reaction
United States Patent US7632641 Note: If you have problems viewing the PDF, please make sure you have the latest version ofAdobe Acrobat. Back to full text