为研究HY5基因在拟南芥分枝发育方面的生物学功能,本研究比较了拟南芥突变体hy5⁃215、HY5过表达株系HY5⁃GFP与野生型在分枝数目的差异。如图6所示,突变体hy5⁃215的莲座叶分枝少于野生型,而过表达株系HY5⁃GFP的莲座叶分枝多于野生型。进一步统计分析...
After ChIP, qPCR was used to examine the enrichment of certain DNA fragments with specific primers listed in Supplemental Table 1. All ChIP–qPCR experiments were independently performed in triplicate and representative results are shown. Yeast One-Hybrid Assay For the yeast one-hybrid assay, ...
The full-length coding region of HY5 was PCR-amplified using the primers listed in Table S2, then the product was digested with BamHI and SacI and cloned into pET-32a vectors. The recombinant His-fusion protein was transformed into Escherichia coli BL21 (DE3) and purified following the instr...