其中,基础培养基为无血清IMDM培养基;内胚层细胞诱导分化因子包括EGF,FGF2,丁酸钠和Wnt信号通路激动剂;肝前体细胞诱导分化因子包含FGF2,HGF和ITSX,肝成熟细胞诱导分化因子包含抑瘤素M,地塞米松和ITSX.使用本发明的化学成份明确的体外诱导体系对第4代hWJMSCs细胞进行诱导后,细胞形态呈现成熟肝细胞典型的双核特征,表达上...
目的: 1.采用熔融纺丝法制备60度斜交叠层纤维环支架,表面添加多巴胺改性后评估其理化特性及相容性.2.研究60度斜交叠层支架复合人脐带干细胞(HWJ-MSCs)诱导分化构建组织工程纤维环的可靠性.3.探究60度斜交叠层纤维环支架纤维直径对人脐带干细胞诱导分化的影响效果. 方法: 1.以聚已内酯(PCL)颗粒为材料,通过熔融纺...
Human Wharton's jelly mesenchymal stem cell-conditioned medium (hWJMSCs-CM) possesses anticancer, wound healing, cerebral ischemia, brain/spinal cord injury, and liver failure properties. From the previous study hWJMSCs had anticancer property. The variation of the molecules in secretome of CM is ...
目的:1.采用熔融纺丝法制备60度斜交叠层纤维环支架,表面添加多巴胺改性后评估其理化特性及相容性.2.研究60度斜交叠层支架复合人脐带干细胞(HWJ-MSCs)诱导分化构建组织工程纤维环的可靠性.3.探究60度斜交叠层纤维环支架纤维直径对人脐带干细胞诱导分化的影响效果.方法:1.以聚已内酯(PCL)颗粒为材料,通过熔融纺丝法...
Viability and cytotoxicity of hWJ-MSCs after gold nanoparticles loading.S. N. LeowChi D. LuuM. H. Hairul NizamP. L. MokR. RuhaslizanH. S. WongWan Haslina Wan Abdul HalimM. H. NgB. H. I. RuszymahS. R. Chowdhury
This study aims to examine the antiaging potential of secretome gel (SC) from human Wharton Jelly Mesenchymal Stem Cells (hWJMSCs) in a UVB-induced mice model. Materials and Methods: The secretome was obtained from hWJ-MSCs and made in gel form. Male mice were radiated by UVB for 15 min...
Confocal microscopy of the whole eye injected with hWJ-MSCs.S. N. LeowChi D. LuuM. H. Hairul NizamP. L. MokR. RuhaslizanH. S. WongWan Haslina Wan Abdul HalimM. H. NgB. H. I. RuszymahS. R. Chowdhury
El-Gamel, Nadia E. A.Department of Chemistry, Faculty of Science, Cairo University, Giza, EgyptAli, Said A.Department of Biophysics, Faculty of Science, Cairo University, Giza, EgyptMohamed, Aly FahmyThe International Center for Advanced Researches (ICTAR), Giza, Egypt...