Cell proliferation of THP-1 and HUVECs in monocultures or co-culture after 24 h exposure to CB, measured by the BrdU assay.Yi CaoMartin RoursgaardPernille Høgh DanielsenPeter MøllerSteffen Loft
proliferation assay was used to observe the proliferative capacity of cells in each group on days 1, 3, 5, and 7. Wound healing and Transwell migration assays were used to detect the effect of SCF on cell migration under either direct or indirect coculture conditions, respectively.In vitro...
Rapid and Localized Mechanical Stimulation and Adhesion Assay: TRPM7 Involvement in Calcium Signaling and Cell Adhesion. PLoS ONE, 10(5): e0126440. Ramcharan, K. 2015. The Endotheliome and the Angiome in Colorectal Cancer.MD Thesis, U Birmingham. Tochikubo, J., N. Matsuda, Y. Ota, T....
respectively, of that in OPNpt20-treated cells but not in OPNpt20-SLAA-treated cells (Figure 1e and f). OPNpt20-mediated HUVEC proliferation was confirmed by the MTT assay, and compared with OPNpt20-treated cells, the results showed that cell survival was lower for OPNpt20-RAA- and OP...
MECcellヾerived exosomes were obtained and coヽultured with human umbilical vein endothelial cells (HUVECs), then the capabilities of cell proliferation, metastasis and angiogenesis of HUVECs were measured by CCK wound healing, transwell and tube formation assay, respectively. Chloride intracellular ...
(NC inhibitor)由Genepharma公司合成,miR-552和U6引物序列由上海生工生物工程有限公司设计合成,Lipofectamine 2000脂质体转染试剂盒购自美国Thermo- fisher公司,噻唑蓝(MTT)试剂盒购自索莱宝生物科技有限公司,放射免疫沉淀分析(radioimmuno-precipitation assay,RIPA)蛋白裂解液、增强化学发光(enhanced chemiluminecence,ECL...
cell(HUVEC)proliferationandtubeformationinvitroandunderstanditsacti onpathway.Methods:MTTmethodwasusedtO accesstheeffeCtofarrestenatdifferentconcentrationsontheproliferationof HUVEC.CapillaryformationwasdetectedbyMa- trigel—plugassayinvitro.Results:MTTassayshowedthatarresteninhibited ...
miR-22 was discovered to regulate cell proliferation and apoptosis in response to environmental toxicants. We have reported that endosulfan can cause endothelial toxicity in human umbilical vein endothelial cells (HUVECs). In the present study, we investigated the involvement of miR-22 in endosulfan...
analyzetheresults.ResultsTheMTTassaydemonstratedthatBreviscapineenhancedtheproliferationofHUVEC.Thereweresignificant diferenceswhencomparedwiththecontrolgroup(0/ xl/m1) <0.05).ConclusionOurresultsindicatesstimulatingcellproliferationmay beasapotentialmechanismsthatBreviscapinecantreatthediabeticperipheralneuropathyeffectively...
μmol/L),tanshinol sodium(1.56-200 μmol/L),paeoniflorin (1.56-200 μ mol/L),formononetin(1.56-200 μ mol/L),salvianolic acid B(1.56-200 μ mol/L),catechin(1.56-200 μ mol/L)and astragaloside Ⅳ(1.56-200 μmol/L)] on the proliferation of HUVECs were evaluated by CCK-8 assay....