除了普通的内皮细胞特征例如“鹅卵石状”,VIII因子阳性着色,能摄取乙酰基化低密度脂蛋白,人脐血管内皮细CD31染色阳性。用IL-1或TNF-α预先处理过的细胞也能选择性地表达E-selectin和VCAM。 人脐静脉内皮细胞(HUVEC)提取于人脐带静脉组织,原代冻存。每管含有细胞数>5×105 cells/ml,此细胞通过vWF/Factor VIII 和...
The CD31 staining results showed that both VEGF and HUVEC co-culture could enhance the angiogenesis of rMSCs and hMSCs. However, the proliferation and osteogenic differentiation of MSCs when cultured with VEGF and HUVECs showed a species dependent response. Taken together, VEGF immobilization and co...
(B) Protein expression levels of VEGF-A, VEGFR-2, MMP-2 and MMP-9 in atherosclerotic plaques of ApoE-/- mice were determined by immunohistochemical staining. Bars indicate 50 µm. (C) Representative Immunohistochemically stained images and quantification of CD31 to measure new vessels in ...
(2)分别取出各组大鼠颅骨标本观察缺损处大体生长状况;(3)取出的样本后经过甲醛固定、EDTA脱钙、石蜡包埋、切片机切片后进行苏木精-伊红染色法(Hematoxylin-eosinstaining)染色法染色以及CD31、CD34免疫组化染色观察颅骨缺损处移植支架中新生血管数量,并检测计算微血管密度(Microvessel density,MVD)以及CD34的累积光密度值(...
qRT-PCR exhibited that the optimized scaffold could stimulate angiogenesis associated genes VEGF and CD31 expression. These characterizations indicated that this scaffold can be considered as an ideal candidate for tissue engineering.Zhang, JingDeng, Aipeng...
Methods: In vitro cultured human umbilical vein endothelial cells (HUVECs) were incubated with CRP (20 mg/l) for 24 h. The numbers of EMPs with CD31- and CD51-positive staining were assessed flow-cytometrically, and NO production was measured using the Griess reaction in the presence or ...
The expression of CD31 and vimentin was determined by Immunofluorescence staining and western blot analysis. Notch1 protein level was detected by western blot analysis.Spironolactone significantly prevented TGF-β-stimulated EndMT by down-regulate vimentin and up-regulate CD31 in HUVECs (p<0.01).It ...