2.1.1 Blood flow homogeneity Human blood is not inherently a homogenous substance. Blood is a body fluid composed of numerous kinds of components. Four of the most important components are plasma, red blood cells (RBC/erythrocytes), white blood cells (leukocytes), and platelets. Plasma is respo...
Put the slides onto the stage of each microscope, which is the flat platform under the lenses. Place the slide with the human blood on the stage of one microscope and the slide with the frog blood on the stage of the other microscope. Clip the slides into place using the clips attached ...
The therapeutic effects of mesenchymal stem cells-extracellular vesicles have been proved in many inflammatory animal models. In the current study, we aimed to investigate the effect of extracellular vesicles (EVs) derived from human umbilical cord-MSC (hUCSC-EV) on the clinical score and inflammato...
Eight weeks’ post transplantation, the BM of recipient mice were comprehensively analyzed via flow cytometry (Fig.4A). Both BM and PB showed engraftment of human cells at a rate of 52.7 ± 35.1% and 18.2 ± 12.6%, respectively. 9.1 ± 6.5% of BM-resident human cells expr...
The optical device is capable of detecting the side- and forward-scattered light of white blood cells, with sufficient resolution for the discrimination of monocytes and lymphocyte populations in a peripheral blood mononuclear cell (PBMC) sample. Moreover, the immunofluorescent detection of these ...
To remove red blood cells, samples were incubated in red blood cell lysis buffer (15.5 mM NH4Cl, 0.57 mM K2HPO4, 0.01 mM EDTA × 2 H2O in PBS) for 6 minutes and subsequently washed with wash buffer. Samples were divided into aliquots containing approximately one million ...
Neurodegenerative diseases (NDs), such as Alzheimer’s disease (AD), are driven by neuroinflammation triggered by activated microglial cells; hence, the phenotypic regulation of these cells is an appealing target for intervention. Human adipose tissue-derived mesenchymal stem cells (hAD-MSCs) may be...
3b. Figure 3c shows an enlargement of the region defined by the white box in panel Fig. 3a corresponding to the cluster of infected cells (Fig. 3d), revealing contact between CD8+ TEM cells and infected epithelial cells. Fig. 3 CD56+ TEM cells contact HCMV-infected decidual glands and ...
In-depth analyses of epithelial cells reveal the presence of ionocytes as a medullary population, while the expression of tissue-specific antigens is mapped to different subsets of epithelial cells. This work thus provides important insight on how the diversity of thymic cells is established, and...
single cells are ordered by infection status (white: non-infected cells,n = 17; black: infected cells,n = 23), and by increasing HCV viral load (blue bar plot on top).cGlobal modulation of the compound-specific gene signatures by HCV infection and drug treatment. Source data ...