Sphingosine kinases are cytosolic or membrane-associated enzymes that catalyze the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P). Two types of sphingosine kinases, SPHK1 and SPHK2, are known to be expressed in human cells. The two enzymes share considerable amino acid sequence simil...
SphK1 activities were valued as pmol/hour/g protein, normalized to the control. Ceramide assay Using a previously described protocol17, the cellular ceramide content was analyzed, and its value was expressed as fmol by nmol of phospholipid. Ceramide content in I-BET726-treated cells was always ...
786-O cells or the primary human RCC cells (“RCC1”) were treated with SC66 (3 μM) for indicated time periods, ROS production (c and k), GSH/GSSG ratio (d), mitochondrial depolarization (e and l), SphK1 expression and activity (f), as well as the ceramide contents (g and ...
2b). It was shown previously that S1P and TNF signaling are interconnected, as TRAF2 (TNFR-associated factor 2, which is part of the signaling complex of TNFR1) activates SPHK1 and thereby increases S1P. S1P, on the other hand, is an important factor in TNF signaling and canonical NFκB...
(Fig.5e). The three sets of comparisons reveal a highly similar pattern, suggesting that STBD1 W203C mutation leads to the loss of normal functions of STBD1, similar to shSTBD1. Seven out of eight genes that we have examined, including PGK1, ENO2, ALDOC, HK1, PFKP, LDHA, and ALDOA...
Furthermore, densely cultured hADSC spheroid cells with simultaneous red light stimulation secreted an increased amount of angiogenic paracrine factors in the CM. Using ELISA, we confirmed the high concentrations of VEGF and FGF2 in the CM of the Sx7R group. Compared to the S group, the secre...
Exostosin-like 3, Ficolin-1, FosB/GOS3, FRS2, GATA-5, GFAP, GLI-3, HepaCAM, ARNT/HIF-1 beta, IDO, Kallikrein 1, Kell, MDL-1/CLEC5A, NPDC-1, Numb, Olig2, p63/TP73L, Pax3, Semaphorin 4D, Sphingosine Kinase 1/SPHK1, TAZ/WWTR1, TC-PTP, Transglutaminase 3/TGM3, TPST2, ...
Downregulated miR-506 expression facilitates pancreatic cancer progression and chemoresistance via SPHK1/Akt/NF-kappaB signaling. Oncogene 2016; 35: 5501–5514. 15. Nwaeburu CC, Bauer N, Zhao Z, Abukiwan A, Gladkich J, Benner A et al. Up-regulation of microRNA let-7c by quercetin ...
10.1038/ncomms4199 a Th17 TNFi-Th17 b 2.0 IL10 1.8 1.6 IL4 1.4 1.2 1.0 CD160 TGFBR3 0.8 0.6 PROK2 0.4 0.2 SIPA1L2 0.0 C5orf40 –0.2 –0.4 KLRB1 –0.6 –0.8 PCID2 –1.0 CCR6 –1.2 –1.4 LAMP3 –1.6 –1.8 NIPAL4 –2.0 MAMLD1 LGMN IL1R1 IPCEF1 TRIB2 HMOX1 SPHK1 REV3L...
Li J, Wu H, Li W, Yin L, Guo S, Xu Xet al. Downregulated miR-506 expression facilitates pancreatic cancer progression and chemoresistance via SPHK1/Akt/NF-kappaB signaling.Oncogene2016;35: 5501–5514. ArticleCASGoogle Scholar Nwaeburu CC, Bauer N, Zhao Z, Abukiwan A, Gladkich J, Benn...