To investigate the role of the autophagy response to Mtb infection in human macrophages and to reduce inconsistencies associated with changes in the genetic background of the parental strain, we generated two gene-deletion mutants, and cognate complemented strains, in the Mtb H37Rv background. First...
Cell lysates were spun for 20 min at full speed in a pre-chilled centrifuge. The cleared lysate was mixed with Dynabeads Protein G (Thermo Fisher #10003D) pre-conjugated with 10 µg PTBP2 antibody (EMD Millipore #ABE431) and incubated in a 4 °C cold room overnight. Immunoprecipitated...
After mixing the components, 3 consecutive high-speed spins of 20 minutes at 4°C removed traces of glass fibers from the RNA mini column. The Penn Transgenic and Chimeric Mouse Facility injected the mix into the cytoplasm of 200 fertilized C57Bl6 zygotes and incubated them overnight in the...
TCiOss2ufeosrli2d4e hs .aCndellcmellomnoolnaoyelarysewrserweerrienspeedrmtweiacbeilwiziethd PBS, with fixed with 3.7% ice-cold PBS supplemented with 100 mM glycine, 1% BSA and 0.2% Triton X-100 at room temperature for 30 min in a shaker. After three washes with PBS for 5...
From these data, we conclude that the knee joint should produce a torque of 177 Nm in the stance phase, bend 2.7 rad in the swing phase and be able to vary its stiffness as needed within the range of human knee joint stiffness, from 300 Nm/rad at a running speed of 4 m/s to ...
As mentioned above, commuters will not suffer emission exposure when the parking wave speed is lower than the walking speed, i.e., θ < vw. Based on the given parameter values, the condition that commuters have no emission exposure is satisfied at the social optimum since θ = 1 km/h ...
sensors Article Human-In-The-Loop Assessment of an Ultralight, Low-Cost Body Posture Tracking Device Marek Sierotowicz * , Mathilde Connan and Claudio Castellini Institute of Robotics and Mechatronics, German Aerospace Center (DLR), 82234 Weßling, Germany; mathilde.connan@dlr.de (M.C.); ...
The average wind speed was set to 1.5 m/s, based on Yi County records [55]. Prevailing directions of southeast and south were selected to simulate the summer wind environment. The results show that the open space wind speed is relatively high along Lake Nanhu, Lake Yuezhao and along the ...
Generic Task/Activity A Totally unfamiliar, performed at speed with no real idea of likely consequences B Shift/restore system to new or original state on a single attempt without supervisor procedure C Complex task requiring a high level of comprehension and skill D Fairly simple task performed ...
This is because the operator may not be able to see the connection at certain heights, or to manually push and guide the lifted part into its final correct position. Mechanical tools can be classified according to their guiding orientation into two groups: guide pins or similar tools for ...