产品名称:Cas9 Expressing Immortalized Human Skeletal Muscle Cell Line 产品型号: 产品展商:ABM 产品文档:无相关文档 简单介绍 Cas9 Expressing Immortalized Human Skeletal Muscle Cell Line Cas9 Expressing Immortalized Human Skeletal Muscle Cell Line的详细介绍 ...
Most previous studies focused on one particular mechanism or cell type, leaving a gap in our understanding of muscle aging as a whole. To address this, recent mouse and human skeletal muscle studies pioneered the use of either single-cell RNA sequencing (scRNA-seq)12,13,14,15,16or single-n...
In this study, primary human skeletal muscle myoblasts (HSMM) were established as a novel human primary cell line that is highly permissive to CHIKV infection, with maximal amounts of infectious virions observed at 16 hours post infection. Genome-wide microarray profiling analyses were ...
In the present study, we characterized for the first time the excitation-contraction coupling machinery of an immortalized human skeletal muscle cell line. Intracellular Ca2+ measurements showed a normal response to pharmacological activation of the ryanodine receptor, whereas 3D-SIM (superresolution ...
Firstly, we did not treat human primary skeletal muscle cells with serum from NAFLD and ESLD patients or CON individuals, but instead used C2C12 murine cells, therefore this approach does not allow for the potential role of peripheral factors i.e., intrinsic muscular and neural properties. ...
human digestive system;human endocrine system;renal system;skin;human muscle system;nervous system;human reproductive system;human respiration;human sensory reception; andhuman skeletal system. For a description of how the body develops, fromconceptionthroughold age,seeaging;growth;prenatal development; ...
Working with primary cells and cell types capable of differentiation can be particularly challenging. We present a streamlined workflow optimised for investigation of primary human skeletal muscle cells. We applied the workflow to differentiated and undifferentiated cells and we investigated the effect of ...
primary fibers); and (3) adult-type myogenesis (muscle adaptation to applied stimuli and regeneration)38. Signals coming from the dorsal region of the neural tube (WNT1 and delta-like canonical Notch ligand 1, DLL1), specifically from the neural crest cells, activate the expression of muscle...
Skeletal muscle biopsies and primary cell isolations For young adults (n = 9, male, 27 ± 4.4 years-old), skeletal muscle tissue (~ 150 mg) was obtained from the vastus lateralis via a conchotome biopsy. Consent and ethical approval were granted for the collection of muscle tis...
skeletal muscle. While TNFα is thought to promote satellite cell/MPC proliferation59,60, TWEAK has been shown to either promote68,69or inhibit70satellite cell/MPC proliferation. When chronically elevated, both TNFα and TWEAK drive canonical NFκB p50/p65-mediated protein catabolism and muscle ...