Hybridization: First, 100 ng of probes were supplemented with 1 μg of Cot-I DNA (Invitrogen) and 3 μg of Sheared Salmon Sperm DNA (Invitrogen). After precipitation, the probes were resuspended in deionized formamide (Sigma-Aldrich), denatured for 7 min at 75 °C and further...
CD46 is common in thymocytes, T cells, B cells, monocytes, granulocytes, NK cells, plaets, endothelial cells, epithelial cells, fibroblasts, placenta and sperm. Description CD46 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 302 amino acids (35-...
In the case of dot blotting hybridization, 6 μg of human COT-1 DNA (Roche Molecular Biochemicals) per milliliter and 0.2× SSC were also added to the hybridization solution. The bolt of MTE Array2 was washed four times in 2× SSC containing 1% SDS at 65 °C and twice in 0.1× SSC...
After dehydration through a cold ethanol series, the chromosomes were incubated with heat-denatured probe (500 ng of each), 3 μg of Cot-1 DNA (Gibco BRL), and 7 μg of salmon sperm DNA in 10 μl of 50% formamide/10% dextran sulfate/2× SSC for 16–18 hours at 37°C. Post...
Briefly, P1 DNA (P1-12423) was labeled with biotin-14-dATP, precipitated with human Cot 1 DNA, dissolved in hybridization solution (2× SSC (1× SSC = 0.15 m NaCl and 0.015m sodium citrate), 10% dextran sulfate, 1% Tween 20, and 50% formamide, pH 7.0), and heat-denatured. Slides...
The presence of parasite proteins, such as PfEMP1 and other adhesins on the surface of erythrocytes, causes these infected cells to sequester to endothelia and placenta via interactions with surface receptor molecules such as CD36, chondroitin sulfate A, ICAM-1 and selectins, resulting in the ...
Interestingly, in a guinea pig model, after infection and treatment, measurable amounts of the compound were detected in fetal blood, attesting that the drug is capable of crossing the placenta in pregnant animals [213]. BAY 38-4766 is in clinical development and has shown a pos...
The CDH13, ATP2C2, and TLDC1 genes exhibited tissue-specific imprinting, however, COTL1, CRISLPLD2, and GSE1 escaped imprinting. Four DMRs, established after fertilization, were found in this region. Two DMRs were located between the ZDHHC7 and KIAA0513 genes, and two were in exon 1 of...
For microarray-based hybridization, standard “high stringency conditions” are defined as hybridization in 50% formamide, 5× SSC, 0.2 μg/μl poly(dA), 0.2 μg/μl human cot1 DNA, and 0.5% SDS, in a humid oven at 42° C. overnight, followed by successive washes of the microarray ...
FIG. 4: Northern blot analysis of poly(A)+ RNA from 20 human tissues: pancreas (lane 1), kidney (2), skeletal muscle (3), liver (4), lung (5), placenta (6), brain (7), heart (8), leukocyte (9), colon (10), small intestine (11), ovary (12), testis (13), prostate (...