A DNA having an hPGC-1 promoter activity; a method of screening a substance modifying this promoter activity; and drugs, which contain substances modifying the promoter activity as the active ingredient, for activating mitochondria and treating obesity and/or diabetes based on the activation of ...
We have now cloned and characterized the human PGC-1α-b promoter. The muscle-specific transcription factors MyoD and MRF4 transactivated this promoter through interaction with a proximal E-box motif. Furthermore, either forced expression of Ca2+- and calmodulin-dependent protein kinase IV (Ca...
1. The importance of the cardiac energy metabolism in healthy and diseased heart 2. Morphological changes of the heart after birth – perinatal developmental hypertrophy 3. Changes in energy metabolism as hallmarks of perinatal maturation of CM – benchmarking hiPSC-derived CM 4. Methods to mature...
Here we report that SNPs in the brain-specific promoter region of the transcriptional co-activator PGC-1, a master regulator of metabolism, modulate age of onset and survival in two large and independent ALS populations and this occurs in a strictly male-specific manner. In complementary animal ...
2a). In addition, although the gene expressions of pancreatic digestive enzymes were lost in cultured acinar organoids, the promoter methylation patterns for many of them were maintained (Figs. 2b, S2e). Of the 5410 fresh acinar hypomethylated sites with a beta value difference of at least ...
The human HES1 promoter sequence was inserted into the firefly luciferase vector. Following UVB exposure, HDFs were incubated with FM, AMSC-EV, HUMSC-EV, TIMP1, GI254023X or VPA, as previously described. Then, HDFs were transfected with human-HES1-promoter-pGL3-Basic together with Renilla pRL...
To knock-down expression of p53 and p16/p19, short hairpin RNAs against p53 (#1224) and p16/p19 (#478) were inserted in a miR-30 backbone driven by the MSCV promoter (Premsrirut et al., 2011). Organoid infections were performed as described previously (Koo et al., 2012). In ...
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significantly repressed the upregulation of δ-catenin-luciferase activities induced by E2F1. Hes1 binds directly to the E-boxes onδ-cateninpromoter and can reduce the expression of δ-catenin in prostate cancer cells. In prostate cancer CWR22-Rv1 and PC3 cell lines, which showed distinct δ-...
S9b). We identified three regions proximal to the DNMT3B promoter that showed enrichment for ZIC2 in primed hESCs and that are selectively open in primed hESCs. Among these, two candidate enhancers showed reduced BRG1 occupancy in ZIC2−/−ZIC3−/− hESCs (Supplementary Fig. S9c). ...