PBMCs of Monkey-#5 post-transplant (8 wpt) alone (Null) or incubated with CD3 antibody were applied as negative and positive control. h, Number of spots (left) detected in IFN-γ ELISpot assay and cytokine activity (right) of various incubation conditions as quantified by ELISpot reader ...
PBMC or isolated T- or NK-cell preparations were used to humanize mice by single or multiple i.v. injections. CDX and PDX from different entities were s.c. (i.e. lymphoma) and/or i.v. (leukemia) transplanted on those humanized mice. Tumor growth of orthotopic models was followed up ...
Studies have shown that tumor growth of triple negative breast cancer (TNBC) CDX as well as TNBC and non-small cell lung cancer (NSCLC) PDX – after being transplanted into HIS mice and treated with another anti-PD-1 antibody, Pembrolizumab (Keytruda) – was significantly delayed, mirroring ...
Human pluripotent stem-cell-derived islets (hPSC-islets) are a promising cell resource for diabetes treatment1,2. However, this therapeutic strategy has not been systematically assessed in large animal models physiologically similar to humans, such as no
Human challenge model HIV: Human immunodeficiency virus HLA: Human leukocyte antigen HSC: Hematopoietic stem cell Ig: Immunoglobulin KI: Knock-in MHC: Major histocompatibility complex NHP: Nonhuman primate PBMC: Peripheral blood mononuclear cell
1b). The PBMC-iPSCs also passed the PluriTest assay with high pluripotency and low novelty scores (Supplementary Fig. 1c) and maintained normal cytogenetic status (Supplementary Fig. 1d). OriP/EBNA1 vectors replicate just once per cell cycle, and in the absence of drug selection, the ...
Monitoring CDX2 expression using CDX2-GFP H9 ESCs (Figure S3L), CDX2-GFP+ cells lost GFP expression after 7 or 10 days in TE induction medium, CT induction medium, or other conditions except for A83-01, CHIR99021, and lysophosphatidic acid (ACL), which was applied for mouse polar-like...
Atoh-1- expressing cells differentiate into secretory cells, whereas absorptive cells arise from Atoh-1-independent cells that express Hes-1 and ultimately Caudal-related homeoprotein-2 (CDX2). Beyond its critical roles in normal development and homeostasis of the gut, Notch signaling may be ...
consequently, the lack of NK-derived IFN-γ in their model, resulted in downregulation of OXPHOS and RP gene expression of a variety of immune cells, particularly myeloid cells, infiltrating tumor (Supplementary Fig.11d). These results suggest that one switching mechanism could be the IFN-γ ...
Digestive system diseases arise primarily through the interplay of genetic and environmental influences; there is an urgent need in elucidating the pathogenic mechanisms of these diseases and deploy personalized treatments. Traditional and long-established model systems rarely reproduce either tissue complexity...