Highly permissive to human xenograft, including primary or established tumor cells (CDX or PDXs) by virtue of the SIRPαNODallele expression Lifespan after humanization similar to that of the wild-type mouse⇨supports long-term engraftment studies ...
PBMC or isolated T- or NK-cell preparations were used to humanize mice by single or multiple i.v. injections. CDX and PDX from different entities were s.c. (i.e. lymphoma) and/or i.v. (leukemia) transplanted on those humanized mice. Tumor growth of orthotopic models was followed up ...
PBMCs of Monkey-#5 post-transplant (8 wpt) alone (Null) or incubated with CD3 antibody were applied as negative and positive control. h, Number of spots (left) detected in IFN-γ ELISpot assay and cytokine activity (right) of various incubation conditions as quantified by ELISpot reader ...
Human pluripotent stem-cell-derived islets (hPSC-islets) are a promising cell resource for diabetes treatment1,2. However, this therapeutic strategy has not been systematically assessed in large animal models physiologically similar to humans, such as no
S2B). Furthermore, the microfluidic culture seemed to increase the general differentiation state of IEC at the expense of proliferation as noted by the upregulation of expression of transcription factors involved in differentiation programs HES1, CDX2 and KLF4 and a decrease of expression of stem ...
Digestive system diseases arise primarily through the interplay of genetic and environmental influences; there is an urgent need in elucidating the pathogenic mechanisms of these diseases and deploy personalized treatments. Traditional and long-established model systems rarely reproduce either tissue complexity...
Monitoring CDX2 expression using CDX2-GFP H9 ESCs (Figure S3L), CDX2-GFP+ cells lost GFP expression after 7 or 10 days in TE induction medium, CT induction medium, or other conditions except for A83-01, CHIR99021, and lysophosphatidic acid (ACL), which was applied for mouse polar-like...
1b). The PBMC-iPSCs also passed the PluriTest assay with high pluripotency and low novelty scores (Supplementary Fig. 1c) and maintained normal cytogenetic status (Supplementary Fig. 1d). OriP/EBNA1 vectors replicate just once per cell cycle, and in the absence of drug selection, the ...
BRACHYURY and CDX2 mediate BMP-induced differentiation of human and mouse pluripotent stem cells into embryonic and extraembryonic lineages Cell Stem Cell, 9 (2011), pp. 144-155 View PDFView articleView in ScopusGoogle Scholar Bernstein et al., 2006 B.E. Bernstein, T.S. Mikkelsen, X. Xie,...
Directed biomechanical compressive forces enhance fusion efficiency in model placental trophoblast cultures Prabu Karthick Parameshwar Chen Li Christopher Moraes Scientific Reports (2024) KAT8-mediated H4K16ac is essential for sustaining trophoblast self-renewal and proliferation via regulating CDX2 Shil...