Understanding the human de novo mutation (DNM) rate requires complete sequence information 1 . Here using five complementary short-read and long-read sequencing technologies, we phased and assembled more than 95
Transfer RNA (tRNA) is present at tens of millions of transcripts in a human cell and is the most abundant RNA in moles among all cellular RNAs. tRNA is also the most extensively modified RNA with, on an average, 13 modifications per molecule. The p
(FPKM). One of the principal units of RNA abundance in the context of RNA sequencing experiments, defined as the number of sequenced fragments per kilobase of annotation per million mapped fragments. Oligonucleotide capture A method for enriching cDNA libraries with sequences of interest using solut...
recently reported the generation of transgenic macaque monkeys harboring CAG promoter-driven APP transgenes with Swedish, Artic (E618 G in APP695; E693G in APP770) and Iberian mutation (I641F) (Seita et al., 2020), whose high aggregation activity was validated in the mutation knock-in ...
We found the median false positive mutation rate to be 0.000256% per (Enge et al., 2017). Fusion detection from scRNaseq Fusion transcripts were detected with STAR-fusion (Haas et al., 2019) (https://github.com/STAR-Fusion/STAR-Fusion/wiki) version 1.6.0, run from a Docker container ...
(Nwia et al.2022). Studies in mouse models including the recent generation of an inducible intestinal epithelial-cell-specificNhe3knockout mouse model mimicking congenital sodium diarrhea has yielded valuable information regarding the important role of NHE3 on intestinal epithelial integrity and its ...
Generation of functional human pancreatic beta cells in vitro. Cell. 2014;159(2):428–39. Article CAS PubMed PubMed Central Google Scholar Parent AV, Faleo G, Chavez J, Saxton M, Berrios DI, Kerper NR, et al. Selective deletion of human leukocyte antigens protects stem cell-derived ...
Parasite progression and growth assay General assay description To assess the effect of inhibitors on the ring to schizont progression, and growth from ring stage to next generation ring stage, a tightly Percoll-synchronised culture with about 2% p...
Generation and characterization of PSB205. A The principle of MabPair technology for producing two correctly assembled antibodies from a single mammalian cell line. Top panel, co-expression of two different antibodies in a single production cell line requires the simultaneous introduction of DNAs encodi...
The mutation rate was assumed to be 2.0 × 10−8 bp per generation. This hybrid model has features of two published demographic histories31, 32 and has been shown to recapitulate the number and frequency distributions of both rare and common synonymous sites, as well as empirically observed...