2.2. Human IL-15 ELISA The Human IL-15 Quantikine ELISA Kit (Cat#D1500 R&D Systems, Minneapolis, MN, USA) was used to quantify levels of human IL-15 in the sera of Hu-NSG-Tg(IL-15) and humanized NSG mice according to the manufacturer's guidelines, and optical density was determined...
These data, obtained by indirectly measuring VEGF activity, were strongly corroborated by the direct measurement of VEGF levels in conditioned RBE4 media as detected by ELISA. Altogether, these findings highlighted a novel role of sub-toxic concentrations of human amylin in promoting the secretion of...