most of which are known to be affected by O-glycosylation either because glycosylation interferes with or blocks binding to the protein core (e.g., mAb to MUC1 such as SM3 or 5E10)28or because O-glycans are required for the binding (e.g., mAbs to Tn-MUC1 (5E5), Tn-...
As a control, IgG antibodies were used, which did not inhibit the migration or invasion at all (data not shown). Values represent means of at least five independent experiments ±s.e.m. Significant differences are indicated in the graph *Po0.05. Inhibition of IL-8, bFGF, TNF-a and VEGF...
3、抗体IgG样品中应不含BSA、甘氨酸等成分。 4、大分子蛋白应提供分子量、等电点、缓冲液成分及pH等信息;小分子多肽需提供氨基酸序列;小分子化合物还需提供分子结构式。 产品信息: 产品名称 Recombinant Human CD3 zeta 规格 50μg, 200μg, 1mg 种属 ...
anti-rabbit IgG Alexa Fluor 555 Thermo Fisher Scientific Cat# A-21429, RRID:AB_2535850 PE-Dazzle-594- Human PD-L1 (CD274) antibody (clone 29E.2A3) BioLegend Cat# 329732, RRID:AB_2616889 PE-Dazzle-594-Mouse IgG2b, κ Isotype Ctrl Antibody BioLegend Cat# 400358 PE-Human STAT1 (clone ...
Total IgG in patient CSF and serum was determined using a hu- man IgG ELISA kit following manufacturer's instruc- tions (Immunology Consultants Laboratory, Inc.). Human MOG constructs Serum IgG binding to full-length native human MOG α1 isoform (native-MOG), a native MOG mutant P42S (...
The following mouse antihuman MoAb were used: Fluorescein isothiocyanate (FITC)-conjugated anti-CD4 (SK3, IgG1), anti-CD25 (2A3, IgG1), anti-CD14 (MΦP9, IgG2a), and anti-HLA-DR (L243, IgG2a), pure and FITC-conjugated anti-CD28 (L293, IgG1), and phycoerythrin (PE)-conjugated anti...
C. Cells were transfected with miR-374b mimic for 24 h, and then incubated with CCL3 (10 ng/mL), and the VEGF-A expression was detected by ELISA kit. D. The culture medium were collected as CM and then applied to EPCs for 24 h. The capillary-like structure formation in EPCs was ...
The horseradish peroxidase-conjugated goat anti-human IgG (Fab specific) was obtained from Sigma, St Louis, MO, USA. The competitive ELISA kit (20080526) was purchased from the Veterinary Institute of the Academy of Military Medical Sciences, Changchun, China. The Kunming mice were provided by ...
(Fig.2G). Human TNFα was not detected in serum from naive hNSG mice or non-humanized NSG mice injected with LPS. We also detected human IgG and IgM antibodies in blood from hNSG mice but not non-humanized NSG mice (Fig.2H,I). Together, these data prove that human immune cells ...
After washing, rabbit anti-polyvalant human IL-2 was added to the wells and finally probed with alkaline-phosphatase-conjugated goat anti-rabbit immunoglobulin G (IgG). The bound specific anti-IL-2 antibody was quantitated colorimetrically by an enzymatic reaction after addition of substrate para...