After cultivation in BD Difco™ Brain Heart Infusion (BHI) broth (Fisher Scientific, USA) under 5% microaerobic conditions (5% oxygen) at 37 °C for 24 h, the S. mutans cells were spread on BHI agar (Fisher Scientific, USA) to form one million colony-forming units (CFU). On ...
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Second, the IC50 values obtained from TSPO2-expressing RBC membranes, as compared to those obtained with TSPO1-enriched rat heart microsomes [28], were one-order lower for both NCS1016 (0.45 vs. 0.058 µM, respectively) and NCS1026 (0.28 vs. 0.01 µM, respectively), supporting the ...