To address these gaps in knowledge, we perform an integrative analysis of resting and inflamed leukocytes from humans and mice and assess the degree of conservation of gene expression. We find that human and mouse transcriptomes can be analyzed together and that lineage-specific gene expression was ...
Results Design of TPC2 pore-only proteins. We designed constructs encoding each of the pore regions of human TPC2 (S5-S6 and S11-S12) (Fig. 1B). To define the pore boundaries, a multiple sequence alignment was performed with the pore regions of previously characterised pore-only and...
import the results file into the ProQuantum cloud-based software at https://apps.thermofisher.com/apps/proquantum. Using this software, the data can be analyzed easily to obtain protein concentration values. The software allows you to set up standard curves, design plate layouts, set up ...
A.R.Hanson, inColour Design (Second Edition), 2012 Abstract Colour is incredible! It is an important sense providing us with essential information about our world, but it is not simple. This chapter describes how we resolve light the way we do, through an attempt to create a space to con...
Probe library design for RNA MERFISH and chromatin tracing We selected 40-bp target sequences for DNA or RNA hybridization by considering each contiguous 40-bp subsequence of each target of interest (the mRNA of a targeted gene or the genomic locus of interest) and then filtering out off-targe...
The cDNA library was diluted to a final concentration of 2.25 nM (200 μl volume) and sequenced on 2× SP flow cells of Illumina NovaSeq 6000. Space Ranger (version 1.1.0, 10x Genomics) was used for the read-mapping to the human reference genome (GRCh38) with default parameters. ...
This difficulty has led to the design of pumps containing two reservoirs for keeping the prandial insulin separate from the amylin derivative. The US patent US2016/001002 from the company ROCHE describes such a pump, in order to enable the co-administration of amylin and of a prandial insulin ...
Proximal epithelia develop because they are located further away from distally located FGF reservoirs in the mesenchyme, in a mechanism that appears dependent on concentration gradients [64]. BMP4 plays a key role in lung bud formation from foregut endoderm and establishment of both dorsoventral (...
Transcription factors (TFs) control specificity and activity of gene transcription, but whether a relationship between these two features exists is unclear. Here we provide evidence for an evolutionary trade-off between the activity and specificity in human TFs encoded as submaximal dispersion of aromati...
structure of the human CaV3.1 channel was resolved. However, the structural basis of modulation mechanism(s) of the current clinically used drugs, as mentioned above, remains elusive, yet such information should be of great importance for the deployment of next-generation therapeutic agents specifical...