based on its location within the body. The dermal fibroblast also has the unique title of being the first human somatic cell to be induced into a pluripotent stem cell line[2,3].Dermal Fibroblasts (HDF) are the most prevalent cell in human dermis, and one of the most important architects...
Human Dermal Fibroblast: A Promising Cellular Model to Study Biological Mechanisms of Major Depression and Antidepressant Drug ResponseHuman dermal fibroblastshuman skin fibroblastsmajor depressionmajor depressive episodeantidepressant drugcellular modelBackground: Human dermal fibroblasts (HDF) can be used as ...
Dermal fibroblasts cell line was purchased as primary cell line from the ATCC (American Type Culture Collection, Manassas, VA, USA). Primary keloid fibroblast cell line were obtained from human keloid explant under IRB protocol. Both cells were used for experiment after 2~3 passage. Full size ...
品名:Human Dermal Fibroblast-adult cell (HDF-a)成人真皮成纤维原代细胞 规格:每管含有细胞数>5x10^5cells/ml 产品描述: 成纤维细胞是来源于中胚层的一种间充质细胞。它被广泛应用于细胞和分子生物学的研究。这主要是因为它是一种 较容易培养的,耐受性较好的细胞,可应用于基因转染、显微注射等多种操作。现已...
1) Human dermal fibroblast 人真皮成纤维细胞 1. Methods Human dermal fibroblasts were isolated from prepuce by enzymatic digestion with 0. 结果胰酶消化法成功培养出人真皮原代成纤维细胞,纯度达99%;人真皮成纤维细胞高表达PAR1、PAR3 mRNA及蛋白,微弱表达PAR2 mRNA及蛋白,不表达PAR4 mRNA及蛋白。 2. ...
2.1. Cell culture/exposure Primary adult human fibroblast were acquired from Cascade Biologics (Carlsbad, CA), sub-cultured and maintained according to the supplier's protocol. Cells were grown in Medium 106 supplemented with a low serum growth supplement (LSGS) Kit, both purchased from Gibco (...
产品名称:Immortalized Human Dermal Fibroblast-fetal Feeder Cells 产品型号: 产品展商:ABM 产品文档:无相关文档 简单介绍 Immortalized Human Dermal Fibroblast-fetal Feeder Cells Immortalized Human Dermal Fibroblast-fetal Feeder Cells的详细介绍 Print Version ...
Cell line, herbs and reagents Human dermal fibroblasts (HDFs) were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA) and cultured in Fibroblast Growth Kit-Low serum (ATCC PCS-201-041) containing 2% fetal bovine serum, 2 mM l-glutamate, and 50 U/ml penicillin (Sigma...
Contraction forces produced by single, human dermal fibroblasts (HDF), cultured on deformable silicone substrata, were quantified using video microscopy and image analysis. Cell contraction causes deformation of the substrate, which appears as a series of surface wrinkles perpendicular to the long axis ...
dermal stem cell; DSC; fibroblast; Geneticin; selective detachment; immunomagnetic cell separation; MACS®; EasySep™; positive selection; negative selection1. Introduction Melanoma is the most aggressive and most lethal form of skin cancer. The identity of the original target cell, which acquires...