Single-cell transcriptomic analyses of human and mouse cortex show an increased diversity of glutamatergic neuron types in supragranular cortex in human and pronounced gradients as a function of cortical depth. To probe the functional and anatomical correlates of this transcriptomic diversity, we ...
Human neurons in autaptic culture. (A)Schematic diagram of the autaptic culture protocol for hiNs. IPSCs were plated and infected with Ngn2, rtTA and eGFP on day −1, followed by application of doxycycline to start Ngn2 expression and puromycin selection on day 0 to 3. HiNs were co-cult...
Taken together, these results show that the transplanted small human organoids survived, became postmitotic after one month and differentiated into cortical neurons in multiple layers in vivo. Engrafted organoids differentiated into functionally mature neurons in the host brain To examine the electrophysio...
Functional cortical neurons and astrocytes from human pluripotent stem cells in 3D culture Nat. Methods, 12 (2015), pp. 671-678 CrossrefView in ScopusGoogle Scholar Penkowa et al., 1999 M. Penkowa, J. Carrasco, M. Giralt, T. Moos, J. Hidalgo CNS wound healing is severely depressed in...
cortical neuronsevolutionhuman cortexhuman pyramidal neuronsintelligenceHuman cognitive abilities are generally thought to arise from cortical expansion over the course of human brain evolution. In addition to increased neuron numbers, this cortical expansion might be driven by adaptations in the properties ...
Thus CTX0E16 neurons can be used to study cortical cell function, and furthermore, as these neurons express a range of disease-associated genes, they represent an ideal platform with which to investigate neurodevelopmental mechanisms in native human cells in health and disease....
Thus CTX0E16 neurons can be used to study cortical cell function, and furthermore, as these neurons express a range of disease-associated genes, they represent an ideal platform with which to investigate neurodevelopmental mechanisms in native human cells in health and disease....
N. et al. Integrative functional genomic analyses implicate specific molecular pathways and circuits in autism. Cell 155, 1008–1021 (2013). Article CAS Google Scholar Willsey, A. J. et al. Coexpression networks implicate human midfetal deep cortical projection neurons in the pathogenesis of ...
in the scRNA-seq dataset (n = 75,497 cells) for all control and C9 ALI-COs at 150 DIV using cell-marker genes15. ULN, upper layer cortical neuron; L4, layer 4 ULN; DLN, deep layer neuron; IN, interneurons; imN, immature neurons; IP, intermediate progenitors; OL/OPC, ...
Surprisingly, we found that FGF13 is highly expressed in a subset of human cortical neurons through to adulthood, in particular in several IN subtypes, including ID2, LAMP5-NOS1, PV-SCUBE3, and PV (Figure 3B). To validate this protracted FGF13 expression, we focused on NDNF+ ID2s, ...