To further define the multipotency of JNK-IN-8-expanded cells, we performed the colony-forming unit (CFU) assay. We found that JNK-IN-8 can also increase the colony-forming unit of granulocyte, erythrocyte, macrophage, and megakaryocyte (CFU-GEMM) by about four folds, on day 10 (Fig.2...
Human bone marrow fibroblast colony-forming units (CFU- F). Prog Clin Biol Res. 1984; 154: 209-36Castro-Malaspina H, Ebell W, Wang S, et al. 1984; Human bone marrow fibroblast colony-forming units (CFU-F). Prog Clin Biol Res 154: 209-236....
Colony forming unit assay Human clonogenic progenitor assays were performed by plat- ing 50 000 cells from day 15 and day 22 hEBs into methylcellu- www.cell-research.com | Cell Research npg Developmental impact of MLL-AF4 in hESC fate 1000 lose H4230 (Stem Cell Technologies, Vancouver, ...
Bone marrow (BM)-derived mesenchymal stromal/stem cells (MSCs), which is characterized by non-hematopoietic, plastic-adherent, colony-forming cells, were first identified by Friedenstein and colleagues decades ago. Despite their complexity and heterogeneity, evidences increasingly support the notion that...
Limiting dilution orthotopic injections were used to evaluate tumor initiation, while serial colony-forming unit, reconstitution and tumorsphere assays were performed to assess self-renewal and differentiation. Pulse-chase bromodeoxyuridine (5-bromo-2-deoxyuridine [BrdU]) labeling was used to examine cell...
Human Bone Marrow Quality Control Kit Quality control kit for hematopoietic colony assays Fresh Cord Blood Proficiency Testing Program For the Hematopoietic Colony Forming Unit (CFU) Assay Uante,您的科研物资战略合作伙伴! 重庆市寰宇安泰生物科技有限公司...
Single-cell colony-forming unit (sc-CFU) was performed on fetal HSCs, as previously described51. Single, live, Lin−, CD34+, CD38−, CD62L+, CD52+cells isolated from the fetal liver of three different fetuses with Ts21 (median of 13 PCW) and disomy (median of 12 PCW) were inde...
CFU-eCFU-cErythropoiesisHemopoietic microen-vironmentTo clarify the relationship between fibroblastoid colony-forming units (CFU-f) and hemopoietic precursor cells, simultaneous assays of CFU-f, CFU-e, BFU-e, and CFU-c from the bone marrow of 10 healthy volunteers were performed. The number ...
cells were subjected to in vitro colony forming unit (CFU) assays. Edited cells retained their clonogenic potential and produced similar frequencies of erythroid and myeloid cells without lineage skewing compared to controls while yielding similar number of colonies of mock-treated cells (Fig.1k). Up...
Hematopoietic cell assays For CFC assays, 400 sort-purified CD133+CD34+or CD133lowCD34+cells were transferred into 1 ml MethoCult H4434 (StemCell Technologies, Vancouver, Canada). Hematopoietic colonies were scored after 14 days discriminating colony forming unit- (CFU-) macrophage (M), granulo...