Human Cell-Free Protein Expression System是利用人源细胞提取物的无细胞蛋白质合成系统。本系统中的Cell Lysate包含了所有体外蛋白质合成的必需因子(核糖体、翻译起始/延伸因子和tRNA等)。只需将携带靶基因的pT7-IRES Vector、T7 RNA Polymerase、Mixture-3(氨基酸和ATP等)和Mixture-2(翻译增强因子)加入到Cell Lysa...
protein. Any target gene cloned into the MCS in frame is transcribed as an RNA-containing-EMCV IRES under the control of the T7 promoter. Efficient high level protein synthesis can be performed in the Human Cell-Free Protein Expression System, due to the effect of the EMCV IRES, ...
protein expressioncell activationimmunologyHistorically, some of the first cell-free protein expression systems studied in vitro translation in various human blood cells. However, because of limited knowledge of eukaryotic translation and the advancement of cell line development, interest in these systems ...
Recent research into the use of cell-free expression systems for UCP overproduction has identified it as a suitable alternative to conventional recombinant expression hosts. Rat UCP1 has been successfully expressed in a cell-free system in the presence of a fluorinated surfactant[45]and initial cell...
protein ligands to the cell-free expression system: (i) co-expression or (ii) co-addition. For co-expression, we added varying amounts of a second expression plasmid to the cell-free expression system to constitutively produce the protein of interest. For co-addition, we directly added the ...
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Keywords Cell-free expression Human endothelin system G-protein coupled receptor Nanodisc Lipid screening Vasoactive peptide Sorry, something went wrong. Please try again and make sure cookies are enabledReferences [1] D.M. Rosenbaum, S.G.F. Rasmussen, B.K. Kobilka The structure and function of...
Our activator ZK53 not only selectively activates HsClpP other than bacterial ClpPs but also shows significant antiproliferative activity in LUSC cell lines. ZK53 does not have a fused-ring system (like imipridones ONC201 and ONC212) or macrocyclic peptide scaffold (like ADEP 4), which is ...
Thus, our screen has identified conserved coPARSE-lncRNAs regulating cancer cell proliferation. Functional validation of the conservation of lncRNA homologs We next explored the functional conservation of coPARSE-lncRNAs using a CRISPR–Cas12a KO-rescue system, in which KO human lncRNAs were ...
A large-scale molecular interaction network of protein-protein interactions (PPIs) enables the automatic detection of molecular functional modules through a computational approach. However, the functional modules that are typically detected by topologica