国际细胞系认证委员会 (ICLAC, International Cell Line Authentication Committee)于2012年成立。它的成立旨在提高学术圈对细胞鉴定的认知,帮助科研工作者减少对错误/被污染细胞系的使用。目前,与ICLAC合作的组织/机构有ATCC、Cellosaurus、CCTCC、ECACC、JCRB等多家细胞库或细胞系数据库,因此ICLAC发布的相关指南与建议十分...
McLaren, RS, Reid, Y, Storts, DR (2013) Human cell line authentication: the critical first step in any project using human cell lines. Methods Mol Biol 963: pp. 341-53MCLAREN RS,REID Y,STORTS DR.Human cell line authentication: the critical first step in any project using human cell ...
Figure 1. Workflows for cell line authentication. Two methods are available for cell line authentication. (A) Cells can be spotted onto NUCLEIC-CARD devices, punches of the cards amplified directly using the CLA Identifiler Direct kit, and fragments analyzed on A...
In this webinar, we describe a complete workflow for human cell line authentication by combining the Applied Biosystems AmpFLSTR Identifiler PCR Amplification Kit, gold-standard capillary electrophoresis (CE)–based instrumentation and GeneMapper analysis software. ...
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Here we evaluate a panel of 96 single-nucleotide polymorphism (SNP) assays utilizing Fluidigm microfluidics technology for authentication and sex determination of human cell lines. The SNPtrace Panel was tested on 907 human cell lines. Pairwise comparison of these data show the SNPtrace Panel ...
In modern biology, the correct identification of cell types is required for the developmental study of tissues and organs and the production of functional cells for cell therapies and disease modeling. For decades, cell types have been defined on the bas
et al. Match criteria for human cell line authentication: where do we draw the line? Int. J. Cancer 132, 2510–2519 (2013). Article CAS PubMed Google Scholar Baker, D. et al. Detecting genetic mosaicism in cultures of human pluripotent stem cells. Stem Cell Rep. 7, 998–1012 (2016...
cell line; unintended immortalization of non-malignant B cells by 'passenger' Epstein-Barr virus (EBV) leads to the establishment of B-lymphoblastoid cell lines (termed EBV+ B-LCLs), an event which is much more frequent than the establishment of a 'true' leukemia-lymphoma-myeloma cell line....
(FBS, Gibico, #10270106), 1% Penicillin/Streptomycin (P/S, Gibico, #15070063) in a humidified atmosphere of 5% CO2at 37 °C. Cell line identity was confirmed by the Multiplex human cell line Authentication Test (MCA, Mutiplexion) or Cell Line Authentication (GENEWIZ, Suzhou). We ...