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The proteins in the membranes were detected with Luminata Crescendo Western HRP substrate (Merck KGaA, Darmstadt, Germany) via an Amersham Imager 600 (GE Healthcare, Chicago, IL, USA). Protein expression was analysed via ImageJ software (National Institutes of Health, Bethesda, USA). Structure ...
Some of these data can be generated within a traditional clinical context, but much of it will come from the day-to-day life of people outside of conventional medical research or clinical care settings. The analysis of data gathered through this study will allow for previously disparate ...
We thank Himanshu Bhat and Thomas Benner (Siemens Healthcare) and Steven Cauley (MGH) for modifying and supplying the simultaneous multislice-BOLD imaging sequence. This work was supported by NIH EY028163 (S.L.). Author information Authors and Affiliations Department of Psychological and Brain ...
Firstly, we set the root and the end-point clusters with ‘getLineages’ function, and then we calculated the principal curves (‘getCurves’ function), the pseudotime estimates (‘slingPseudotime’ function) and the lineage assignment weights (‘slingCurveWeights’ function). To identify ...
Although the immunological- and cytokine-mediated events preceding the development of full-blown Type 1 diabetes in the nonobese diabetic (NOD) mouse model are becoming better known, the events preceding disease in humans are less well-characterized. In
Here we represent human lives in a way that shares structural similarity to language, and we exploit this similarity to adapt natural language processing techniques to examine the evolution and predictability of human lives based on detailed event sequen
Adjuvants are key components of many vaccines, used to enhance the level and breadth of the immune response to a target antigen, thereby enhancing protection from the associated disease. In recent years, advances in our understanding of the innate and ad
The composite mesh was manually punched multi-poles in order to promote tissue infiltration and get the close appearance as PP mesh (Fig. 9a0). The implanted rabbits were raised normally after the operation. The wound on the skin cured and the fur regenerated completely after one month post-...
Image acquisition and analysis were performed on an IN Cell Analyzer workstation (GE Healthcare) using a multitarget analysis algorithm to quantify fluorescence intensity in nuclear and cytoplasmic compartments (in total and ppERK1/2 image channels). Conflict of interest The authors declare no ...