The shape and size of observed nanoparticles in the isolated material from human specimens taxes the limits of modern analytical instrumentation but may reflect an end-stage product of plastic degradation that i
The sheep brain and human brain are very similar in overall structure, as are all mammalian brains. Each contains a cerebrum, cerebellum, and brain stem. The sheep brain is smaller, weighing around 140 grams, or about one-tenth of the weight of an adult human brain, though it is still l...
This means that brain-coX is uniquely suited towards improving our understanding of normal regulation throughout brain development. Unfortunately our effort to also make use of brain regions was thwarted due to the large inconsist- encies in brain anatomical annotation between the different datasets ...
For isolating single cells from fresh brain tissue samples (n = 31), we utilized the Adult Brain Dissociation Kit (Miltenyi Biotech, #130107677), which is particularly suited for processing live tissue. This kit enables the enzymatic breakdown of the tissue while maintaining the integrity and...
but targeted to diseases originating in the brain. brain-coX also allows insights into the temporal complexity of the human brain within an easy to use web-tool. This means that brain-coX is uniquely suited towards improving our understanding of normal regulation throughout brain development. Unfor...
Mouse models, although useful for certain functional studies, are not particularly suited for neurodegeneration related functional genomic studies due to the differences in gene expression [94] (e.g. lacking certain AD-associated genes) and aging between human and mouse microglia [95]. The ...
Olah et al. [27] identified a set of genes that are specifically expressed in subsets of microglia freshly purified from aged postmortem human brains. Remarkably, all four of our iMG clusters expressed these microglial genes, includingC1Q, CABLES1, TREM2, ITGAM, PROS1, APOE, SLCO2B1, HAVCR2...
Human evolution is hypothesized to be driven primarily by changes in gene regulation rather than protein sequence divergence [12,13,14], highlighting the transcriptome as an appropriate nexus for investigating evolution, as demonstrated by recent studies assessing gene expression in brains across multiple...
We found that an average of 87.45% of reads were uniquely mapped. There was a higher percentage of reads mapping to intronic regions in nuclear samples compared to the corresponding cytoplasmic samples. This was expected due to the higher levels of pre-mRNA in the nuclear fraction. Intronic ...
Studies have shown that at a structural level and even at a neurochemical level human and non-human primate brains continue to undergo regulated developmental changes at least through puberty and likely much longer (Gogtay et al, 2006; Levitt, 2003; Lewis, 1997). At a molecular level ...