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TheKcell types represent the cell types for which we deconvolute signal and obtain amount estimates. Note, this is the "column" dimension in matrixYand the length of the vector represented byπ. Funding sources NIMH (USA) grantR01 MH123183 ...
sgRNA sequences were cloned into a pNGx-LV-g003 vector8 to generate the individual lentiviruses (Supplementary Data 6). HEK293FT cells were plated in 6-well plates (Greiner, 657160) for a 50–70% confluency on the day of transfection. For each sgRNA, 1.5 µl TransIT and 55.1 ...
After culturing overnight, the medium was removed and the cells were transfected with 80 ng per well of either of the firefly constructs together with 40 ng per well of pRL-TK (reporter vector for normalization using Renilla luciferase controlled by the herpes simplex virus thymidine kinase ...