Next, we labeled each electrode based on annotations provided by Talairach Demon78. We analyzed the EMG signals using the bipolar configuration, notch filtering (60 and 120 Hz) the signals, band-pass filtering them between 20 and 170 Hz, and computed the envelope by taking the absolute ...
For organoid sections, the number of nuclei labeled with Hoechst in each field was counted and recorded as the total cell number. In the transplanted brain sections, the number of grafted cells labeled with human nuclei (hN) was counted and recorded as the total grafted cell number. More ...
Phosphatase assays were carried out using 32P-labeled hyperphosphorylated tau as a substrate. Hyperphosphorylation of recombinant human brain tau352 was performed by using 100 000 × g brain extract from a 20 day-old rat as the source of protein kinases as described previously [5], [40]. The...
The comparison of the results generated with the immunohistochemistry protocols used in this study (Figure2) [23] led to a better understanding of the distribution of neurogenesis markers in the brain cytoarchitecture. The labeled streptavidin-biotin method was suitable for the detection of nestin in...
Astrocytes with multiple long fiber-like processes were labeled as fibrous astrocytes, and astrocytes with complex processes branching from thicker stem branches are labeled as protoplasmic astrocytes. The primate-specific interlaminar astrocytes were identified by cell soma in the marginal zone and long,...
HTAD integrates Discriminative Active Learning (DAL), an effective supervised learning approach that trains a binary classifier to discriminate between the labeled or unlabeled samples [22]. This implementation is complemented by a web-based labeling tool, collectively forming an effective strategy for ...
To quantify the bioavailability of rhMANF after intranasal delivery, we measured rhMANF levels in the brain and blood using 125I-labeled rhMANF and hMANF ELISA (Additional file 1: Fig. 2). In the dMCAo rats, approximately 0.4% (222 CPM/g) of the radioactivity of 125I-labeled rhMANF ...
A. Fluorescence energy-transfer dye-labeled primers for DNA sequencing and analysis. Proc. Natl Acad. Sci. USA 92, 4347–4351 (1995). Article ADS CAS PubMed PubMed Central Google Scholar Lee, L. G. et al. New energy transfer dyes for DNA sequencing. Nucleic Acids Res. 25, 2816–...
We next carried out direct experimental verification of the neuronal cell type assignment by the lipidome assessment of fluorescently labeled pyramidal neurons and unlabeled cells isolated using cell sorting from the brain of Thy1-ChR2-YFP45 transgenic mice (Fig. 5g; Supplementary Fig. 10). The ...
we also labeled whole brain structures on the LFB sections of this block with a semi-automated approach78, and deformed them along with the histology. The figure displays the alignment of the different brain structures across MRI and histology, both in the sectioning (coronal) and reconstructed ...