In this research, we investigated the effects of human growth hormone(hGH) on aging via evaluation of telomerase activity.Materials and Methods: Primary human foreskin fibroblast cells were isolated, culturedand treated with different concentrations of hGH. BrdU and MTT cell proliferation assaysand ...
notably active during infectious diseases; evidence suggests that they are specifically tasked with controlling self-reactive T cells that have been activated by infection8. Thus, at least two major types of regulatory T (Treg) cell maintain tolerance, but their specific roles have not been explored...
Limited understanding exists regarding how aging impacts the cellular and molecular aspects of the human ovary. This study combines single-cell RNA sequencing and spatial transcriptomics to systematically characterize human ovarian aging. Spatiotemporal
Brain Aging Center, UCSF, San Francisco, USA Kaitlin Cassaleto & Joel Kramer Contributions D. K. performed the research that is shown in Figs.1,2,3,4,5, and6and Supplementary Figs. 1, 2, 4, 6, 7, and 9; analyzed the data, composed these figures; and co-wrote the manuscript. D...
Elisa Majounie & Andrew B. Singleton Computational Biology Core, Laboratory of Neurogenetics, National Institute on Aging, National Institutes of Health, Bethesda, MD, USA Jinhui Ding & J. Raphael Gibbs Genetic Technologies Group, Laboratory of Neurogenetics, National Institute on Aging, National Inst...
Across the globe, 2-3% of humans carry the p.Ser132Pro single nucleotide polymorphism in MLKL, the terminal effector protein of the inflammatory form of programmed cell death, necroptosis. Here we show that this substitution confers a gain in necroptotic
S3). Subsequently, the expression of secretory factors and extracellular matrix-related genes was validated by ELISA and qRT-PCR in UC-MSCs with different LRP1 expression levels. Consistent with the analysis of single-cell data, the mRNA expression of CXCL1, CXCL8, IL1β, CCL2, FGF7, ...
Autocrine growth stimulation of human keratinocytes by epidermal cell-derived thymocyte-activating factor: implications for skin aging Keratinocytes in serum-free medium secreted large amounts of ETAF, as reported previously, and keratinocyte cultures derived from newborn donors secreted ... DN Sauder,BM...
future research can incorporate humanized mice into experimental plans to better understand how SCI, or other types of neurological injury and disease, affect human immune system functionin vivo. In this context, humanized mice provide a unique tool in the translational research toolbox and should fa...
(Supplementary Table1). All individuals fulfilled definite or probable Bohan and Peter classification criteria for dermatomyositis. Anti-TIF1 autoantibody positivity was identified by immunoprecipitation, and confirmed by ELISA, as described previously51. Samples were selected at random and based on plasma...