INTRODUCTION...1 PRINCIPLE OF THE ASSAY...
Pro-MMP-9 is an inactive proenzyme secreted by multiple cell types. The N-terminal pro region is proteolytically removed, resulting in active MMP-9 which can degrade collagens and elastin as well as several non-ECM molecules. Long Name ...
Stem cell therapies have been widely investigated for their healing effects. However, the translation of these therapies has been hampered by the requirement to deliver live allogeneic or autologous cells directly to the wound in a clinical setting. Mult
PEX pro-360 Recombinant Human C-terminal hemopexin-like domain of MMP-2 (445-635 a.a.) PEX19 pro-925 Recombinant Human Peroxisomal Biogenesis Factor 19 PFDN1 pro-277 Recombinant Human Prefoldin Subunit 1 PFDN2 pro-001 Recombinant Human Prefoldin Subunit 2 PFDN4 pro-092 Recombinant Human Prefol...
Here, we reported that hAM preparations (homogenate and extract) inhibited the expression of the epithelial–mesenchymal transition markers N-cadherin and MMP-2 in bladder cancer urothelial cells in a dose-dependent manner, while increasing the secretion of TIMP-2. Moreover, hAM homogenate exerted ...
Scar formation is an intractable medical problem that appears after skin wounds have healed. Recent research has shown that exosomes secreted by human adipose mesenchymal stem cells (ASC-Exos) can benefit wound healing. To further explore the therapeutic
(Fig.5b). Therefore, MP1 were defined as proinflammatory macrophages. MP2, characterized as profibrotic macrophages, was marked by secreted phosphoprotein 1 (SPP1)and matrix metallopeptidase 9 (MMP9)8(Fig.4b, c). MP3 was defined as antifibrotic macrophages expressing folate receptor beta (FLOR2...
Immunofluorescent staining of KLK12 and fibronectin in the capillary formation assay Capillary formation assay was performed as previously described6. Briefly, ECs and fibroblasts were seeded together (20,000 cells/well of each cell type) in a 24-well plate in complete EC medium. After 9 days ...
Immunofluorescent images shown here were used for qualitative observations only. Full size image The MMP is generated upon the passing of electrons through a group of mitochondrial protein complexes I, III, and IV located on the inner mitochondrial membrane, which in turn is used to drive ...
Human MMP1 activity To determine the activity of human MMP1, cell culture supernatants were collected after 48 h of culture. The activity of human MMP1 were measured by a SensoLyte® Plus 520 MMP-1 Assay Kit (AnaSpec, San Jose, CA, USA; AS-72012) according to the manufacturer’s ...