Immunofluorescence Confocal Microscopy Cells were grown on coverslips and then stained with a polyclonal antibody raised against pure mature recombinant Omi protein and a mouse anti-Smac monoclonal antibody after fixing the cells with 4% paraformaldehyde. Fluorescein isothiocyanate-conjugated anti-rabbit and...
B, subcellular localization of AREL1 (green) was assessed in H1299 cells by immunofluorescence staining with polyclonal anti-AREL1 serum (1:100). DAPI (blue) and MitoTracker (red) were used to stain nuclei and mitochondria, respectively. Scale bar is 100 μm. C, AREL1 in the cytosolic ...
Cells were incubated overnight with primary antibody (anti-HtrA2/Omi rabbit polyclonal IgG (15775-1-AP, Proteintech) for parental or HtrA2/Omi-deficient cells, anti-HtrA2/Omi rabbit polyclonal IgG (AF1458, R&D Systems) for HtrA2/Omi-reconstituted cells, or anti-FLAG M2 mouse monoclonal IgG...