HTERT RPE-1细胞系|人视网膜色素上皮细胞可以用于谷胱甘肽在微波辐射致hTERT-RPE1细胞基因转录中的作用 对HTERT RPE-1细胞系|人视网膜色素上皮细胞细胞和添加5 0μmol·L-1谷胱甘肽(GSH)培养2 4h后的hTERT RPE1细胞进行2 4 5 0MHz,1 0mW·cm-2的微波辐射,并就有关细胞应激和凋亡基因的转录水平进行比较研究。
;细胞生长特性:贴壁;细胞形态特性:详见细胞说明书;相关细胞有:YD-15Cells、RPE-hTERTCells、NIH 3T3细胞 COLO829Cells;细胞背景资料:详见相关文献介绍;细胞传代方法:1:3-1:6传代,2-3天换液1次。;细胞生长特性:贴壁生长;细胞形态特性:成纤维Cells;相关细胞有:HS-695TCells、L-02Cells、H341细胞 SCC 25Cells;...
Gene set enrichment analysis revealed a senescence-like signature with strong induction of p53 and p21 but in the absence of canonical hallmarks of senescence such as 尾-galactosidase positivity, suggesting incomplete cell fate commitment. When scrutinizing the putative drivers of this growth attenuation...
hTERT immortalised cells, Cell Line Analytical Standards at LGC Standards. Over 100,000 Products Online, Explore our Extensive Range and Purchase Easily via our Webshop
通过SDS-PAGE在每个泳道中分离等量的结合蛋白,并使用抗RPE65 (1:5,000)或抗PP2AC (1:5,000) rAb通过蛋白质印迹进行分析。 冈田酸处理和PP2A磷酸酶测定 将冈田酸(适马-奥尔德里奇·斯坦海姆,德国)溶解在DMSO中至最终浓度为1 mM,并加入到同步化的细胞中至最终浓度为1 nM,持续5小时。孵育后,将细胞在PBS中...
We observed that METTL3 knockdown enhanced RPE cell migration and significantly upregulated the EMT markers N-cadherin (encoded by CDH2 ), fibronectin (FN), Snail family transcription repressor (SLUG), and vimentin. However, YTHDF1 knockdown had the opposite effects and decreased both cell ...
Therefore, we are confident that the RPE-hTERT immortalized cells used in our study are valid to test cellular and molecular effects of MYCN induction. Cell seeding and treatment. To evaluate phenotypic and protein changes, RPE1–MYCN-ER cells were plated in 96 wells overnight in three ...
hTERT immortalised cells, Cell Line Analytical Standards at LGC Standards. Over 100,000 Products Online, Explore our Extensive Range and Purchase Easily via our Webshop
RPE cell cultureWe performed measurements to validate damage threshold trends in minimum visible lesion (MVL) studies as a function of spot size for nanosecond laser pulses. At threshold levels, nanosecond pulses produce microcavitation bubbles that expand and collapse ar...
hTERT-RPECells;细胞背景资料:视网膜色素上皮;hTERT永生;女性;细胞传代方法:1:2-1:3传代;每周换液2-3次。;细胞生长特性:贴壁;细胞形态特性:详见细胞说明书;相关细胞有:NPA'87Cells、MEG-01Cells、Adeno-293细胞 SK 1Cells;细胞背景资料:详见相关文献介绍;细胞传代方法:维持细胞浓度在1×105-2×105,每周补液2-...