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(Supplementary Figs.2–4). In contrast, the serotonin-5HT3R-Salipro data set yielded only an asymmetric map at a nominal resolution of 2.8 Å (Supplementary Figs.2–4). For all maps, local resolution varied and was highest at the ECD and lowest at the ICD (Supplementary Figs.3dand...
Chemicals were purchased from various sources: acetonitrile of a gradient-HPLC grade, ammonium formate, ammonium bicarbonate, ammonium acetate, formic acid, acetic acid, standards of glucose, isomaltulose, saccharose, cellobiose, trehalose were from Sigma-Aldrich (Merck, Germa...
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All other consumables were analytical grade. 2.2. Steady-State Fluorescence All measurements were conducted with an ISS–PC1 spectrofluorometer (ISS, Cham- paign, IL, USA) with sample stirring at 37 ◦C. The hCaM M124C-mBBr (1 µM) was incubated in buffer (10 mM of potassium acetate ...